Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinus seed: extraction and purification process

Chemistry Central Journal

Bahareh Tabatabaee Amid²  Hamed Mirhosseini²  Sanja Kostadinović¹ 

[1] Faculty of Agriculture, University “Goce Delcev”, Krste Misirkov bb, 2000, Štip, Macedonia

[2] Department of Food Technology, Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

关键词: Fatty acid composition;    Essential amino acid;    Carbohydrate;    Chemical structure;    Molecular structure;    Durio zibethinus;    Biopolymer;   

Others  :  788059
DOI  :  10.1186/1752-153X-6-117

 received in 2012-07-18, accepted in 2012-10-01,  发布年份 2012

【 摘 要 】

Background

The biological functions of natural biopolymers from plant sources depend on their chemical composition and molecular structure. In addition, the extraction and further processing conditions significantly influence the chemical and molecular structure of the plant biopolymer. The main objective of the present study was to characterize the chemical and molecular structure of a natural biopolymer from Durio zibethinus seed. A size-exclusion chromatography coupled to multi angle laser light-scattering (SEC-MALS) was applied to analyze the molecular weight (Mw), number average molecular weight (Mn), and polydispersity index (Mw/Mn).

Results

The most abundant monosaccharide in the carbohydrate composition of durian seed gum were galactose (48.6-59.9%), glucose (37.1-45.1%), arabinose (0.58-3.41%), and xylose (0.3-3.21%). The predominant fatty acid of the lipid fraction from the durian seed gum were palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:2). The most abundant amino acids of durian seed gum were: leucine (30.9-37.3%), lysine (6.04-8.36%), aspartic acid (6.10-7.19%), glycine (6.07-7.42%), alanine (5.24-6.14%), glutamic acid (5.57-7.09%), valine (4.5-5.50%), proline (3.87-4.81%), serine (4.39-5.18%), threonine (3.44-6.50%), isoleucine (3.30-4.07%), and phenylalanine (3.11-9.04%).

Conclusion

The presence of essential amino acids in the chemical structure of durian seed gum reinforces its nutritional value.

【 授权许可】

   
2012 Amid et al.; licensee Chemistry Central Ltd.

【 图 表 】

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