Energetic and biochemical valorization of cork boiling wastewater by anaerobic digestion

Biotechnology for Biofuels

Isabel Paula Marques²  Luís Gil²  Francesco La Cara¹ 

[1] Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, Napoli 80131, Italia

[2] Laboratório Nacional de Energia e Geologia, I.P., Estrada do Paço do Lumiar 22, Lisboa 1649-038, Portugal

关键词: Enzymes;    Phenols;    Valuable biomolecules;    Methane;    Cork boiling wastewater;    Anaerobic digestion;   

Others  :  792534
DOI  :  10.1186/1754-6834-7-67

 received in 2013-07-15, accepted in 2014-03-31,  发布年份 2014

【 摘 要 】

Background

In addition to energy benefits, anaerobic digestion offers other interesting advantages. The cork industry is of great environmental, economic and social significance in the western Mediterranean region, with Portugal being the world-leading producer and exporter. Cork boiling wastewater (CBW) is a toxic and recalcitrant organic effluent produced by this sector, which constitutes a serious environmental hazard. However, there is no documented research on anaerobic treatment/valorization performed with this effluent. The work presented here was developed with the aim to use the anaerobic digestion process to convert the CBW polluting organic load into an energy carrier gas and valuable molecules for industry.

Results

No lag phases were observed and a methane yield of 0.126 to 0.142 m³ kg^-1 chemical oxygen demand (COD)_added was registered in the mesophilic consortium experiments carried out in batch flasks at 37 ± 1°C. Anaerobic digestion can be advantageously connected to ultrafiltration or electrochemical processes, due to the following: 1) reduction of ellagic acid content and consequent decrease of CBW viscosity; and 2) increase in conductivity after the anaerobic process, avoiding the electrolyte application of the electrochemical process. The improvement of several CBW biochemical features shows that anaerobic digestion may provide additionally useful molecules. The rise in concentration of some of these compounds, belonging to the benzoic acid family (gallic, protocatechuic, vanillic and syringic acids), is responsible for the increase of antiradical activity of the phenolic fraction. Additionally, some enzymatic activity was also observed and while the laccase activity increased in the digested effluent by anaerobiosis, xylanase was formed in the process.

Conclusions

The multidisciplinary approach adopted allowed the valorization of CBW in terms of energy and valuable biomolecules. By exploiting the anaerobic digestion process potential, a novel methodology to toxic and recalcitrant cork processing wastewater was developed.

【 授权许可】

   
2014 Marques et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Associação Portuguesa de Cortiça (APCOR): A Produção Suberícola. Santa Maria de Lamas: APCOR; 2010. http://www.apcor.pt/artigo/289.htm webcite
• [2]Gil L: Cortiça: Produção, Tecnologia e Aplicação. Lisbon: Instituto Nacional de Engenharia, Tecnologia e Inovação; 1998.
• [3]Gil L: Cortiça da Produção à Aplicação. Seixal: Camara Municipal do Seixal; 2005.
• [4]Ré A, Rodrigues AM, Quintino V: Acute toxicity testing with the European estuarine amphipod Corophium multisetosu. Hydrobiologia 2007, 587:89-99.
• [5]Mendonça E, Picado A, Silva L, Anselmo AM: Ecotoxicological evaluation of cork-boiling wastewaters. Ecotoxicol Environ Saf 2007, 66:384-390.
• [6]Bernardo M, Santos A, Cantinho P, Minhalma M: Cork industry wastewater partition by ultra/nanofiltration: a biodegradation and valorisation study. Water Res 2011, 45:904-912.
• [7]Benítez FJ, Acero JL, Leal AI, González M: The use of ultrafiltration and nanofiltration membranes for the purification of cork processing wastewater. J Hazard Mater 2009, 162:1438-1445.
• [8]Benítez FJ, Acero JL, Leal AI: Treatment of wastewaters from the cork process industry by using ultrafiltration membranes. Desalination 2008, 229:156-169.
• [9]Geraldes V, Minhalma M, Pinho MN, Anil A, Ozgunay H, Bitlisli BO, Sari O: Nanofiltration of cork wastewaters and their possible use in leather industry as tanning agents. Pol J Environ Stud 2009, 18:353-357.
• [10]Oliveira J, Nunes M, Santos P, Cantinho P, Minhalma M: Cork processing wastewater treatment/valorisation by nanofiltration. Water Res 2009, 11:224-228.
• [11]Teixeira ARS, Santos JLC, Crespo JG: Sustainable membrane-based process for valorisation of cork boiling wastewaters. Sep Purif Technol 2009, 66:35-44.
• [12]Minhalma M, de Pinho MN: Flocculation/flotation/ultrafiltration integrated process for the treatment of cork processing wastewaters. Environ Sci Technol 2001, 35:4916-4921.
• [13]Benítez FJ, Acero JL, Leal AI, Francisco JR: Ozone and membrane filtration based strategies for the treatment of cork processing wastewaters. J Hazard Mater 2008, 152:373-380.
• [14]Lan BY, Nigmatullin R, Puma GL: Ozonation kinetics of cork-processing water in a bubble column reactor. Water Res 2008, 42:2473-2482.
• [15]Acero JL, Benitez FJ, Beltran De Heredia J, Leal AI: Chemical treatment of cork-processing wastewaters for potential reuse. J Chem Technol Biotechnol 2004, 79:1065-1072.
• [16]Pintor AMA, Vilar VJP, Boaventura RAR: Decontamination of cork wastewaters by solar-photo-Fenton process using cork bleaching wastewater as H2O2 source. Sol Energ 2011, 85:579-587.
• [17]Vilar VJP, Maldonado MI, Oller I, Malato S, Boaventura RAR: Solar treatment of cork boiling and bleaching wastewaters in a pilot plant. Water Res 2009, 43:4050-4062.
• [18]Beltran De Heredia J, Domínguez JR, López R: Treatment of cork process wastewater by a successive chemical-physical methods. J Agric Food Chem 2004, 52:4501-4507.
• [19]del Castilho I, Hernandez P, Lafuente A, Rodriguez-Llorente ID, Caviedes MA, Paiuelo E: Self-bioremediation of cork-processing wastewaters by (chloro) phenol-degrading bacteria immobilised onto residual cork particles. Water Res 2012, 46:1723-1734.
• [20]Dias-Machado M, Madeira LM, Nogales B, Nunes OC, Manaia M: Treatment of cork boiling wastewater using chemical oxidation and biodegradation. Chemosphere 2006, 64:455-461.
• [21]Mendonça E, Pereira P, Martins A, Anselmo AM: Fungal biodegradation and detoxification of cork boiling wastewaters. Eng Life Sci 2004, 4:144-149.
• [22]Benítez FJ, Acero JL, Garcia J, Leal AI: Purification of cork processing wastewaters by ozone, by activated sludge, and by their two sequential applications. Water Res 2003, 37:4081-4090.
• [23]Gonçalves MR, Freitas P, Marques IP: Bioenergy recovery from olive mill effluent in a hybrid reactor. Biomass Bioenergy 2012, 39:253-260.
• [24]Sampaio MA, Gonçalves MR, Marques IP: Anaerobic digestion challenge of raw olive mil wastewater. Bioresour Technol 2011, 100:10810-10818.
• [25]La Cara F, Ionata E, Del Monaco G, Marcolongo L, Gonçalves MR, Marques IP: Olive mill wastewater anaerobically digested: Phenolic compounds with antiradical activity. Chem Eng Trans 2012, 27:325-330.
• [26]Malico I, Arribas Hernández J, Marques IP: From waste to energy. What is the current contribution of the Portuguese biogas? In Portugal: Economic, Political and Social Issues. Edited by Bento-Gonçalves A, Vieira A. Hauppauge, NY: Nova Science Publishers, Inc; 2012.
• [27]Miguel F, Marques IP, Malico I: Biogas in Portugal: status and public policies in an European context. Energy Policy 2012, 43:267-274.
• [28]Santos A, Bernardo M, Vespeira C, Cantinho P, Minhalma M: Cork industry wastewater characterization: assessment of the biodegradability, reuse and of the relationship between BOD, COD and tannins with TOC. Journal of Water Reuse and Desalination 2012, 2:33-39.
• [29]Anselmo AM, Gil L, Mendonça E: Águas residuais de cozedura da cortiça – caracterização e perspectiva ambiental. Água e Ambiente 2001, 35:1-4.
• [30]Marques IP, Goncalves M, Gil L: Cork boiling wastewater management by anaerobic digestion. In Proceedings of the 4th International Conference on Engineering for Waste and Biomass Valorisation. 10-13 September 2012. Edited by Nzihou A, Castro F. Porto, Portugal; 2012:495.
• [31]Chen Y, Cheng JJ, Creamer KS: Inhibition of anaerobic digestion process: a review. Bioresour Technol 2008, 99:4044-4064.
• [32]Yazici R, Sekman E, Top S, Varank G, Bilgili MS: Degradation of phenolic compounds in aerobic and anaerobic landfills: a pilot scale study. Waste Manag Res 2012, 30:1-9.
• [33]Schink B, Philipp B, Müller J: Anaerobic degradation of phenolic compounds. Naturwissenschaften 2000, 87:12-23.
• [34]Field JA, Lettinga G: The effect of oxidative coloration on the methanogenic toxicity and anaerobic biodegradability of phenols. Biol Waste 1989, 29:161-179.
• [35]Marques IP, Correia JP, Gonçalves MR: Process for the complete treatment of effluents of the olive oil mills in two steps. Patent 2012., 105780(applied on 18 June 2012; published on 31 December 2012; granted on 20 August 2013
• [36]Gonçalves MR, Correia JP, Marques IP: Electrochemical mineralization of anaerobically digested olive mill wastewater. Water Res 2012, 46:4217-4225.
• [37]Minhalma M, De Pinho MN: Tannic-membrane interactions on ultrafiltration of cork processing wastewaters. Sep Purif Technol 2001, 22–3:479-488.
• [38]Khadem S, Marles RJ: Monocyclic phenolic acids; hydroxy- and polyhydroxybenzoic acids: occurrence and recent bioactivity studies. Molecules 2010, 15:7985-8005.
• [39]Crognale S, D’Annibale A, Federici F, Fenice M, Quaratino D, Petruccioli M: Olive oil mill wastewater valorisation by fungi. J Chem Tech Biotechnol 2006, 81:1547-1555.
• [40]Marques IP, Ionata E, Marcolongo L, Sampaio M, Del Monaco G, La Cara F: Olive mill wastewaters anaerobic bioreactor effluents: a source of valuable biomolecules and enzymes. In Proceedings of Symposium “Olive Oil Mills Wastes and Environmental Protection”,16 to 18 October 2012. Chania: Soil Science Institute, Athens, Greece; 2012:246-252.
• [41]Colleran E, Concannon F, Golden T, Geoghrgan F, Crumlish B, Killilea E, Henry M, Coates J: Use of methanogenic activity tests to characterize anaerobic sludges, screen for anaerobic biodegradability and determine toxicity thresholds against individual anaerobic trophic groups and species. Water Sci Technol 1992, 25:31-40.
• [42]Coates JD, Coughlan MF, Colleran E: Simple method for the measurement of the hydrogenotrophic methanogenic activity of anaerobic sludges. J Microbiol Methods 1996, 26:237-246.
• [43]American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF): Standard Methods for the Examination of Water and Wastewater. 20th edition. Washington, DC: APHA, AWWA, WEF; 1998.
• [44]Singleton VL, Rossi JA Jr: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology Viticulture 1965, 16:144-158.
• [45]Naffrechoux E, Fachinger C, Suptil J: Diode-array ultraviolet detector for continuous monitoring of water quality. Anal Chim Acta 1992, 270:187-193.
• [46]Foster P, Morris AW: Ultra-violet absorption characteristics of natural waters. Water Res 1974, 8:137-142.
• [47]von Gadow A, Joubert E, Hansmann CF: Comparison of antioxidant activity of aspalathin with that of other plant phenols of Rooibos tea (Aspalathonlinearis), α-tocopherol, BHT and BHA. J Agr Food Chem 1997, 45:632-638.
• [48]Cruz JM, Dominguez JM, Dominguez H, Parajò JC: Antioxidant and antimicrobial effects of extracts from hydrolysates of lignocellulosic materials. J Agric Food Chem 2001, 49:2459-2464.
• [49]Nazzaro M, Barbarisi C, La Cara F, Volpe MG: Chemical and biochemical characterization of an IGP ecotype chestnut subjected to different treatments. Food Chem 2011, 128:930-936.
• [50]Bülow L, Mosbach K: The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Lett 1987, 210:147-152.
• [51]Childs R, Bardsley W: The steady-state kinetics of peroxidase with 2,2’-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid) as chromogen. Biochem J 1975, 145:93-103.
• [52]Morana A, Esposito A, Maurelli L, Ruggiero G, Ionata E, Rossi M, La Cara F: A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius. Protein PeptLett 2008, 15:1017-1021.