Plasma total antioxidant status in horses after 8-hours of road transportation

Acta Veterinaria Scandinavica

Artur Niedźwiedź¹  Krzysztof Kubiak¹  Józef Nicpoń¹ 

[1] Department of Internal Diseases with Clinic for Horses, Dogs and Cats, The Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wrocław, Poland

关键词: Plasma total antioxidant status;    Antioxidants;    Oxidative stress;    Road transport;    Horse;   

Others  :  789278
DOI  :  10.1186/1751-0147-55-58

 received in 2013-03-07, accepted in 2013-08-14,  发布年份 2013

【 摘 要 】

Background

The aim of this study was to investigate the effects of 8-hour road transport on plasma total antioxidant status (PTAS) and general clinical appearance in horses.

Findings

The study was conducted on a group of 60 horses of different breeds aged from 4 to 10 years. Venous blood was collected and a clinical examination was performed immediately before loading horses onto trailers for an 8 hour transport (I), immediately after unloading them from the trailer (II), and after a subsequent 24 hour stall rest (III). The ferric-reducing ability of plasma (FRAP) was used to determine PTAS. The transportation significantly increased respiratory and heart rates. The average PTAS increased during the three subsequent samplings: I: 170 ± 77 (μmol/l) II: 204 ± 70 (μmol/l) III: 221 ± 74 (μmol/l).

Conclusion

Long-distance transport increased the PTAS horses, as well as respiratory and heart rates.

【 授权许可】

   
2013 Niedźwiedź et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Hartung J: Effect of transport on health of farm animals. Vet Res Commun 2003, 27(Suppl 1):525-527.
• [2]Nielsen BL, Dybkjær L, Herskin MS: Road transport of farm animals: effects of journey duration on animal welfare. Anim 2011, 5:415-427.
• [3]Niedźwiedź A, Filipowski H, Służewska-Niedźwiedź M, Nicpoń J: Stress level in horses during transport as a variable in Krefft’s function. Medycyna Wet 2011, 67:270-274.
• [4]Smith BL, Jones JH, Hornof WJ, Miles JA, Longworth KE, Willits NH: Effects of road transport on indices of stress in horses. Equine Vet J 1996, 28:446-454.
• [5]Stull CL: Responses of horses to trailer design, duration, and floor area during commercial transportation to slaughter. J Anim Sci 1999, 77:2925-2933.
• [6]Friend TH, Martin MT, Householder DD, Bushong DM: Stress responses of horses during a long period of transport in a commercial truck. J Am Vet Med Assoc 1998, 212:838-844.
• [7]Chirase NK, Greene LW, Purdy CW, Loan RW, Auvermann BW, Parker DB, Walborg EF Jr, Stevenson DE, Xu Y, Klaunig JE: Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle. Am J Vet Res 2004, 65:860-886.
• [8]Ito S, Hobo S, Eto D, Sato H: Bronchoalveolar lavage for the diagnosis and treatment of pneumonia associated with transport in Thoroughbred racehorses. J Vet Med Sci 2001, 63:1263-1269.
• [9]Ishida N, Hobo S, Takahashi T, Nanbo Y, Sato F, Hasegawa T, Mukoyama H: Chronological changes in superoxide-scavenging ability and lipid peroxide concentration of equine serum due to stress from exercise and transport. Equine Vet J Suppl 1999, 30:430-433.
• [10]Minka SN, Ayo JO: Hydration state of goats transported by road for 12 hours during the hot-dry conditions and the modulating role of ascorbic acid. J Appl Anim Welf Sci 2012, 15:272-288.
• [11]Wernicki A, Urban-Chmiel R, Kankofer M, Mikucki P, Puchalski A, Tokarzewski S: Evaluation of plasma cortisol and TBARS levels in calves after short-term transportation. Revue Méd Vét 2006, 157:30-34.
• [12]Ghiselli A, Serafini M, Natella F, Scaccini C: Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic Biol Med 2000, 29:1106-1114.
• [13]Halliwell B, Gutteridge JM: The definition and measurement of antioxidants in biological systems. Free Radic Biol Med 1995, 18:125-126.
• [14]Bartosz G: Non-enzymatic antioxidant capacity assays: limitations of use in biomedicine. Free Radic Res 2010, 44:711-720.
• [15]Onmaz AC, Van Den Hoven R, Gunes V, Cinar M, Kucuk O: Oxidative stress in horses after a 12-hours transport period. Revue Méd Vét 2011, 162:213-217.
• [16]Benzie IF, Strain JJ: The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996, 239:70-76.
• [17]Fazio E, Medica P, Cravana C, Giacoppo E, Ferlazzo A: Physiological variables of horses after road transport. Anim 2009, 3:1313-1318.
• [18]Schmidt A, Hödl S, Möstl E, Aurich J, Müller J, Aurich C: Cortisol release, heart rate, and heart rate variability in transport-naive horses during repeated road transport. Domest Anim Endocrinol 2010, 39:205-213.
• [19]Leadon D, Waran N, Herholz C, Klay M: Veterinary management of horse transport. Vet Ital 2008, 44:149-163.
• [20]Friend TH: Dehydration, stress, and water consumption of horses during long-distance commercial transport. J Anim Sci 2000, 78:2568-2580.
• [21]Proops L, Burden F, Osthaus B: Social relations in a mixed group of mules, ponies and donkeys reflect differences in equid type. Behav Processes 2012, 90:337-342.
• [22]Niedźwiedź A, Zawadzki M, Filipowski H, Nicpoń J: Influence of 8-hour road transportation on selected physiological parameters in horses. Bull Vet Inst Pulawy 2012, 56:193-197.
• [23]Cywinska A, Witkowski L, Szarska E, Schollenberger A, Winnicka A: Serum amyloid A (SAA) concentration after training sessions in Arabian race and endurance horses. BMC Vet Res 2013, 9:91. BioMed Central Full Text