Characterization of terrestrial cyanobacteria to increase process efficiency in low energy consuming production processes

Sustainable Chemical Processes

Roland Ulber¹  Kai Muffler¹  Sarah Foltz²  Michael Lakatos²  Stephan Kuhne¹ 

[1]Institute of Bioprocess Engineering, University of Kaiserslautern, Kaiserslautern, D-67663, Germany

[2]Experimental Ecology, University of Kaiserslautern, Kaiserslautern, D-67663, Germany

关键词: Photobioreactor;    Light-curve;    Light and temperature variation;    Terrestrial cyanobacteria;    Carotenoid;    Chlorophyll a;   

Others  :  789188
DOI  :  10.1186/2043-7129-1-6

 received in 2013-02-22, accepted in 2013-05-28,  发布年份 2013

【 摘 要 】

Terrestrial cyanobacteria have seldom been used for biotechnological processes, even though they offer great potential for new pharmaceutical products or other value-added substances. Particularly cyanobacteria of xeric habitats are of biotechnological interest, because they tolerate high temperatures, are desiccation-tolerant and feature low water consumption. In addition, the cyanobacteria collected in deserts are able to produce more photoprotective agents than their counterparts from other habitats, because of their genetical preadaptation. In this study, carotenoid and chlorophyll content of two representative terrestrial cyanobacteria strains, i.e. Nostoc muscorum and Leptolyngbya spec. sampled in Columbia (USA) and Soebatsfontein (RSA), were studied after exposure of the strains to different light conditions and cultivation temperatures. A temperature raise from 17°C to 30°C led to an increase of 46% in chlorophyll a content as well as an increase of 39% in carotenoid content of Nostoc muscorum. An irradiation raise from 19 μmol m^-2s^-1 to 125 μmol m^-2s^-1 resulted in an increase of a 10 to 20 times higher chlorophyll content. Additional results from light-curves support the potential future use of terrestrial cyanobacteria within low energy biotechnological processes using a novel type of photobioreactor to reduce the downstream process costs and nutrients needed during the cultivation. Results indicate that especially light intensity optimization currently holds unused potential.

【 授权许可】

   
2013 Kuhne et al.; licensee Chemistry Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Pulz O: Photobioreactors: production systems for phototrophic microorganisms. Appl Microbiol Biotechnol 2001, 57:287-293.
• [2]Putz O, Gross W: Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 2004, 65:635-648.
• [3]Belnap J, Lange OL: Biological Soil Crusts: Structure, Function and Management. Berlin Heidelberg: Springer; 2001.
• [4]Rascher U, Lakatos M, Büdel B, Lüttge U: Photosynthetic field capacity of cyanobacteria of a tropical inselberg of the guiana highlands. Eur J Phycol 2003, 38:247-256.
• [5]Boyd MR, Gustafson KR, McMahon JB, Shoemaker RH, O´Keefe BR, Mori T, Gulakowski RJ, Wu L, Rivera MI, Laurencot CM, Currens MJ, Cardellina JH, Buckheit RW, Nara PL, Pannel LK, Sowder RC, Hender LE: Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development. Antimicrob Agents Chemother 1997, 41:1521-1530.
• [6]Bewley CA, Gustafson KR, Boyd MR, Covell DG, Bax A, Clore GM, Gronenborn AM: Solution structure of cyanovirin-N, a potent HIV-inactivating protein. Nat Struct Biol 1998, 5:571-578.
• [7]Muller-Feuga A, Moal J, Kaas R: The microalgae for aquaculture. In Life feeds in marine aquaculture. Edited by Stottrup JG, McEvoy LA. Oxford: Blackwell; 2003.
• [8]Krinsky NI: Carotenoid protection against oxidation. Pure Appl Chem 1979, 51:649-660.
• [9]Palozza P, Krinsky NI: Antioxidant effects of carotenoids in vivo and in vitro: an overview. Methods Enzymol 1992, 213:403-419.
• [10]Heinrich U, Tronnier H: Systemische Fotoprotektion durch Carotinoide. Zeitschrift für Phytotherapie 2010, 31:185-187.
• [11]Lakatos M, Bilger W, Büdel B: Carotenoid composition of terrestrial cyanobacteria: response to natural light conditions in habitats in Venezuela. Eur J Phycol 2001, 36:367-375.
• [12]Lüttge U, Kluge M, Bauer G: Botanik. Darmstadt: Wiley-VCH; 2005.
• [13]Brougham RW: The Relationship between the Critical Leaf Area, Total Chlorophyll Content, and Maximum Growth-rate of some Pasture and Crop Plants. Ann Bot 1960, 96:463-474.
• [14]Eppley RW, Sloan PR: Growth Rates of Marine Phytoplankton - Correlation with Light Absorption by Cell Chlorophyll Alpha. Physiol Plant 1966, 19(1):47.
• [15]Bilger W, Bohuschke M, Ehling-Schulz M: Annual time courses of the contents of carotenoids and uv-protective pigments in the cyanobacterium nostoc commune. In J. Cramer in Der Gebrueder Borntraeger Verlagsbuchhandlung. Berlin, Germany: E. Schweizerbart'sche Verlagsbuchhandlung: Stuttgart, Germany; 1997.
• [16]Brock TD: Life at high temperatures. Science 1967, 158:1012-1018.
• [17]Potts M: Desiccation Tolerance of Prokaryotes. Microbiol Rev 1994, 58:755-805.
• [18]Vincent WF, Downes MT, Castenholz RW, Howard-Williams C: Community structure and pigment organisation of cyanobacteria dominated microbial mats in Antarctica. Eur J Phycol 1993, 28:213-221.
• [19]Leisner JMR, Bilger W, Czygan FC, Lange OL: Lipophilous carotenoids of cyanobacterial lichens from different habitats, including an extreme desert site. Cryptogamic Botany 1993, 4:74-82.
• [20]Davis R, Aden A, Pienkos PT: Techno-economic analysis of autotrophic microalgae for fuel production. Appl Energ 2011, 88(10):3524-3531.
• [21]Norsker NH, Barbosa MJ, Vermuë MH, Wijffels RH: Microalgal production — A close look at the economics. Biotechnol Adv 2011, 29(1):24-27.
• [22]Kieseler S, Neubauer Y, Zobel N: Ultimate and Proximate Correlations for Estimating the Higher Heating Value of Hydrothermal Solids. Energy Fuel 2013, 27(2):908-918.
• [23]Krause GH, Weis E: Chlorophyll fluorescence and photosynthesis: The basis. Ann Rev Plant Physiol Plant Mol Biol 1991, 42:313-349.
• [24]Bilger W, Schreiber U, Bock M: Determination of the quantum efficiency of photosystem II and of non-photochemical quenching of chlorophyll a fluorescence in the field. Oecologia 1995, 102:425-432.