Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

BMC Biotechnology

Eva-Maria Siepert³  Esther Gartz⁵  Mehmet Kemal Tur²  Heinrich Delbrück⁴  Stefan Barth¹  Jochen Büchs⁵ 

[1] Department of Pharmaceutical Product Development, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Forckenbeckstr 6, 52074, Aachen, Germany

[2] Institute of Pathology, University Hospital Giessen and Marburg GmbH (UKGM), Langhansstr 10, 35392 Giessen, Germany

[3] Department of Experimental Medicine and Immunotherapy, Institute of Applied Medical Engineering, Helmholtz Institute of RWTH Aachen University & Hospital, Pauwelsstr 20, 52074 Aachen, Germany

[4] Institute of Molecular Biotechnology, RWTH Aachen University; c/o Fraunhofer IME, Forckenbeckstr 6, 52074, Aachen, Germany

[5] AVT. Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany

关键词: Small scale fermentation;    Escherichia coli protein expression;    Fluorescence measurement;    Microtiter plate;    On-line monitoring;    Tryptophan tag;   

Others  :  1134849
DOI  :  10.1186/1472-6750-12-65

 received in 2012-04-12, accepted in 2012-08-13,  发布年份 2012

【 摘 要 】

Background

Conventional fluorescent proteins, such as GFP, its derivatives and flavin mononucleotide based fluorescent proteins (FbFPs) are often used as fusion tags for detecting recombinant proteins during cultivation. These reporter tags are state-of-the-art; however, they have some drawbacks, which can make on-line monitoring challenging. It is discussed in the literature that the large molecular size of proteins of the GFP family may stress the host cell metabolism during production. In addition, fluorophore formation of GFP derivatives is oxygen-dependent resulting in a lag-time between expression and fluorescence detection and the maturation of the protein is suppressed under oxygen-limited conditions. On the contrary, FbFPs are also applicable in an oxygen-limited or even anaerobic environment but are still quite large (58% of the size of GFP).

Results

As an alternative to common fluorescent tags we developed five novel tags based on clustered tryptophan residues, called W-tags. They are only 5-11% of the size of GFP. Based on the property of tryptophan to fluoresce in absence of oxygen it is reasonable to assume that the functionality of our W-tags is also given under anaerobic conditions. We fused these W-tags to a recombinant protein model, the anti-CD30 receptor single-chain fragment variable antibody (scFv) Ki-4(scFv) and the anti-MucI single-chain fragment variable M12(scFv). During cultivation in Microtiter plates, the overall tryptophan fluorescence intensity of all cultures was measured on-line for monitoring product formation via the different W-tags. After correlation of the scattered light signal representing biomass concentration and tryptophan fluorescence for the uninduced cultures, the fluorescence originating from the biomass was subtracted from the overall tryptophan signal. The resulting signal, thus, represents the product fluorescence of the tagged and untagged antibody fragments. The product fluorescence signal was increased. Antibodies with W-tags generated stronger signals than the untagged construct.

Conclusions

Our low-molecular-weight W-tags can be used to monitor the production of antibody fragments on-line. The binding specificity of the recombinant fusion protein is not affected, even though the binding activity decreases slightly with increasing number of tryptophan residues in the W-tags. Thus, the newly designed W-tags offer a versatile and generally applicable alternative to current fluorescent fusion tags.

【 授权许可】

   
2012 Siepert et al.; licensee BioMed Central Ltd.

【 图 表 】

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