Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture

BioMedical Engineering OnLine

Pratthana Chomchalao¹  Sutatip Pongcharoen³  Manote Sutheerawattananonda²  Waree Tiyaboonchai¹ 

[1] Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand and the Center of Excellence for Innovation in Chemistry, Commission on Higher Education, Thailand

[2] Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand

[3] Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand

关键词: Chondrocytes;    Scaffolds;    Gelatin;    Collagen;    Fibroin;   

Others  :  797911
DOI  :  10.1186/1475-925X-12-28

 received in 2013-01-23, accepted in 2013-04-01,  发布年份 2013

【 摘 要 】

Background

In our previous study, we successfully developed 3-D scaffolds prepared from silk fibroin (SF), silk fibroin/collagen (SF/C) and silk fibroin/gelatin (SF/G) using a freeze drying technique. The blended construct showed superior mechanical properties to silk fibroin construct. In addition, collagen and gelatin, contain RGD sequences that could facilitate cell attachment and proliferation. Therefore, in this study, the ability of silk fibroin and blended constructs to promote cell adhesion, proliferation and production of extracellular matrix (EMC) were compared.

Methods

Articular chondrocytes were isolated from rat and cultured on the prepared constructs. Then, the cell viability in SF, SF/C and SF/G scaffolds was determined by MTT assay. Cell morphology and distribution were investigated by scanning electron microscopy (SEM) and histological analysis. Moreover, the secretion of extracellular matrix (ECM) by the chondrocytes in 3-D scaffolds was assessed by immunohistochemistry.

Results

Results from MTT assay indicated that the blended SF/C and SF/G scaffolds provided a more favorable environment for chondrocytes attachment and proliferation than that of SF scaffold. In addition, scanning electron micrographs and histological images illustrated higher cell density and distribution in the SF/C and SF/G scaffolds than that in the SF scaffold. Importantly, immunohistochemistry strongly confirmed a greater production of type II collagen and aggrecan, important markers of chondrocytic phenotype, in SF blended scaffolds than that in the SF scaffold.

Conclusion

Addition of collagen and gelatin to SF solution not only improved the mechanical properties of the scaffolds but also provided an effective biomaterial constructs for chondrocyte growth and chondrocytic phenotype maintenance. Therefore, SF/C and SF/G showed a great potential as a desirable biomaterial for cartilage tissue engineering.

【 授权许可】

   
2013 Chomchalao et al.; licensee BioMed Central Ltd.

【 图 表 】

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