Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption

Biotechnology for Biofuels

Dong Liu¹  Yong Chen¹  Feng-Ying Ding¹  Ting Zhao¹  Jing-Lan Wu¹  Ting Guo¹  Heng-Fei Ren¹  Bing-Bing Li¹  Huan-Qing Niu¹  Zhi Cao¹  Xiao-Qing Lin¹  Jing-Jing Xie¹  Xue-Jun He¹  Han-Jie Ying¹ 

[1] State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 30, Puzhu South Road, Nanjing 211816, China

关键词: Redox modulation;    Adsorption;    Acetoin;    Simultaneous product recovery;    Clostridium acetobutylicum;    Biofilm reactor;   

Others  :  794096
DOI  :  10.1186/1754-6834-7-5

 received in 2013-10-06, accepted in 2013-12-24,  发布年份 2014

【 摘 要 】

Background

Clostridium acetobutylicum can propagate on fibrous matrices and form biofilms that have improved butanol tolerance and a high fermentation rate and can be repeatedly used. Previously, a novel macroporous resin, KA-I, was synthesized in our laboratory and was demonstrated to be a good adsorbent with high selectivity and capacity for butanol recovery from a model solution. Based on these results, we aimed to develop a process integrating a biofilm reactor with simultaneous product recovery using the KA-I resin to maximize the production efficiency of biobutanol.

Results

KA-I showed great affinity for butanol and butyrate and could selectively enhance acetoin production at the expense of acetone during the fermentation. The biofilm reactor exhibited high productivity with considerably low broth turbidity during repeated batch fermentations. By maintaining the butanol level above 6.5 g/L in the biofilm reactor, butyrate adsorption by the KA-I resin was effectively reduced. Co-adsorption of acetone by the resin improved the fermentation performance. By redox modulation with methyl viologen (MV), the butanol-acetone ratio and the total product yield increased. An equivalent solvent titer of 96.5 to 130.7 g/L was achieved with a productivity of 1.0 to 1.5 g · L^-1 · h^-1. The solvent concentration and productivity increased by 4 to 6-fold and 3 to 5-fold, respectively, compared to traditional batch fermentation using planktonic culture.

Conclusions

Compared to the conventional process, the integrated process dramatically improved the productivity and reduced the energy consumption as well as water usage in biobutanol production. While genetic engineering focuses on strain improvement to enhance butanol production, process development can fully exploit the productivity of a strain and maximize the production efficiency.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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