Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2

Biotechnology for Biofuels

Vera Lúcia dos Santos⁵  Ezequias Pessoa de Siqueira²  Daniel Bonoto Gonçalves⁴  Ivana Lula¹  Marcus VD Souza³  Vitor Souza Domingues⁵  Andrea de Souza Monteiro³ 

[1]Laboratório de Ressonância Magnética Nuclear – Dept. de Química, Instituto de Ciências Exatas e Tecnológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

[2]Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou Fundação Oswaldo Cruz, 30190-002, Belo Horizonte, MG, Brazil

[3]Laboratório de Pesquisa em Microbiologia – Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, Brazil

[4]Laboratório de Processos Bioquímicos, Universidade Federal de São João Del-Rei, Campus Centro-Oeste Dona Lindu, Divinópolis, MG, Brazil

[5]Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 31270-901, Belo Horizonte, MG, Brazil

关键词: Diatomaceous earth;    Biodiesel residue;    Response surface methodology;    Yeasts;    Bioemulsifier;   

Others  :  798303
DOI  :  10.1186/1754-6834-5-29

 received in 2012-01-18, accepted in 2012-05-06,  发布年份 2012

【 摘 要 】

Background

The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes.

Results

Trichosporon mycotoxinivorans CLA2 is bioemulsifier-producing yeast strain isolated from effluents of the dairy industry, with ability to emulsify different hydrophobic substrates. Bioemulsifier production (mg/L) and the emulsifying activity (E₂₄) of this strain were optimized by response surface methodology using mineral minimal medium containing refinery waste as the carbon source, which consisted of diatomaceous earth impregnated with esters from filters used in biodiesel purification. The highest bioemulsifier production occurred in mineral minimal medium containing 75 g/L biodiesel residue and 5 g/L ammonium sulfate. The highest emulsifying activity was obtained in medium containing 58 g/L biodiesel refinery residue and 4.6 g/L ammonium sulfate, and under these conditions, the model estimated an emulsifying activity of 85%. Gas chromatography and mass spectrometry analysis suggested a bioemulsifier molecule consisting of monosaccharides, predominantly xylose and mannose, and a long chain aliphatic groups composed of octadecanoic acid and hexadecanoic acid at concentrations of 48.01% and 43.16%, respectively. The carbohydrate composition as determined by GC-MS of their alditol acetate derivatives showed a larger ratio of xylose (49.27%), mannose (39.91%), and glucose (10.81%). ¹ H NMR spectra confirmed by COSY suggested high molecular weight, polymeric pattern, presence of monosaccharide’s and long chain aliphatic groups in the bioemulsifier molecule.

Conclusions

The biodiesel residue is an economical substrate, therefore seems to be very promising for the low-cost production of active emulsifiers in the emulsification of aromatics, aliphatic hydrocarbons, and kerosene.

【 授权许可】

   
2012 Monteiro et al; licensee BioMed Central Ltd.

【 图 表 】

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