Occurrence of diarrhoea and intestinal pathogens in non-medicated nursery pigs

Acta Veterinaria Scandinavica

Ken Steen Pedersen¹  Christian Fink Hansen¹  Lise-Lotte Pedersen¹  Alex Stricker Jakobsen¹  Jens Peter Nielsen¹  Nicolai Weber¹ 

[1]Department of Large Animal Sciences, HERD-Centre for Herd-oriented Education, Research and Development, University of Copenhagen, Groennegaardsvej 2, Frederiksberg C, 1870, Denmark

关键词: Pigs;    Batch medication;    Diarrhoea;    E. coli;    Brachyspira pilosicoli;    Lawsonia intracellularis;   

Others  :  1228607
DOI  :  10.1186/s13028-015-0156-5

 received in 2015-07-03, accepted in 2015-09-24,  发布年份 2015

【 摘 要 】

Background

Intestinal disease in nursery pigs is the most common cause of antibiotic usage in pigs in Denmark. The decision to initiate batch medication of intestinal diseases in nursery pigs is typically made by the stock personnel based on clinical assessments of pigs and counting of diarrhoeic faecal pools on the pen floor. The target population of this study was batches of nursery pigs (10–66 days after weaning) where the stock personnel assessed the pigs to be without signs of intestinal disease and therefore did not needed treatment. The objective was to determine the within-herd prevalence of diarrhoea, and to determine the prevalence of Escherichia coli F4 and F18, Lawsonia intracellularis and Brachyspira pilosicoli by quantitative PCR in pigs with and without diarrhoea.

Results

The overall apparent prevalence of diarrhoeic pigs across sixteen herds was 32.6 % (CI 95 % 27.9–37.3). The prevalence of diarrhoea increased (p ≤ 0.001) with age of the pigs (days after weaning) with an odds ratio of 1.04 (CI 95 % 1.02–1.05) per extra day. Diarrhoeic pools were observed in 51 % of the pens. L. intracellularis, B. pilosicoli, E. coli F4 and F18 were detected in 20, 17, 13 and 11 % of the 256 faecal samples analysed by quantitative PCR respectively. There was no association between detection of pathogens and diarrhoea status of the individual pigs and between detection of pathogens in a pen and diarrhoea floor pools. In 51 % of the samples from diarrhoeic pigs, pathogens were not detected. Only 5 % of the 3060 pigs examined had clinical signs of diseases other than diarrhoea.

Conclusions

One-third of non-medicated nursery pigs had diarrhoea when clinically examined even though they were assessed as healthy by stock personnel. Diarrhoeic status of the pigs and diarrhoeic pools in pen was a poor indicator of intestinal infections with E. coli F4 and F18, L. intracellularis and B. pilosicoli and subclinical infections were common. Therefore, clinical examination and counting of diarrhoea pools should be supported by microbiological testing as decision tools for initiation of batch treatments of intestinal infections in nursery pigs.

【 授权许可】

   
2015 Weber et al.

【 参考文献 】

• [1]Collignon P, Powers JH, Chiller TM, Aidara-Kane A, Aarestrup FM. World Health Organization ranking of antimicrobials according to their importance in human medicine: a critical step for developing risk management strategies for the use of antimicrobials in food production animals. Clin Infect Dis. 2009; 49:132-141.
• [2]Statens Serum Institut, National Veterinary Institute, National Food Institute. DANMAP 2013—use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark. http://www.danmap.org2014. Accessed 1 Dec 2014.
• [3]Stege H, Bager F, Jacobsen E, Thougaard A. VETSTAT—the Danish system for surveillance of the veterinary use of drugs for production animals. Prev Vet Med. 2003; 57:105-115.
• [4]Andreasen M, Alban L, Dahl J, Nielsen AC. Risk-mitigation for antimicrobial resistance in Danish swine herds at a national level. J Agr Sci Tech. 2012; A2:412-416.
• [5]Alban L, Dahl J, Andreasen M, Petersen JV, Sandberg M. Possible impact of the “yellow card” antimicrobial scheme on meat inspection lesions in Danish finisher pigs. Prev Vet Med. 2013; 108:334-341.
• [6]Hybschmann GK, Ersboll AK, Vigre H, Baadsgaard NP, Houe H. Herd-level risk factors for antimicrobial demanding gastrointestinal diseases in Danish herds with finisher pigs: a register-based study. Prev Vet Med. 2011; 98:190-197.
• [7]Pedersen KS, Okholm E, Johansen M, Angen Ø, Jorsal SE, Nielsen JP et al.. Clinical utility and performance of sock sampling in weaner pig diarrhoea. Prev Vet Med. 2015; 120:313-320.
• [8]Frydendahl K. Prevalence of serogroups and virulence genes in Escherichia coli associated with postweaning diarrhoea and edema disease in pigs and a comparison of diagnostic approaches. Vet Microbiol. 2002; 85:169-182.
• [9]Fairbrother JM, Gyles CL. Colibaccilosis. In: Diseases of swine. 0 Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, editors. Wiley, USA; 2012: p.723-749.
• [10]Jacobson M, af Segerstad CH, Gunnarsson A, Fellstrom C, Klingenberg KD, Wallgren P et al.. Diarrhoea in the growing pig—a comparison of clinical, morphological and microbial findings between animals from good and poor performance herds. Res Vet Sci. 2003; 74:163-169.
• [11]Aarestrup FM, Oliver Duran C, Burch DG. Antimicrobial resistance in swine production. Anim Health Res Rev. 2008; 9:135-148.
• [12]Pedersen KS, Johansen M, Angen O, Jorsal SE, Nielsen JP, Jensen TK et al.. Herd diagnosis of low pathogen diarrhoea in growing pigs—a pilot study. Irish Vet J. 2014; 67:24. BioMed Central Full Text
• [13]Stata. Stata Statistical Software. 13 ed. College Station, Texas, USA: StataCorp LP; 2013.
• [14]Pedersen KS, Toft N. Intra- and inter-observer agreement when using a descriptive classification scale for clinical assessment of faecal consistency in growing pigs. Prev Vet Med. 2011; 98:288-291.
• [15]Pedersen KS, Stege H, Nielsen JP. Evaluation of a microwave method for dry matter determination in faecal samples from weaned pigs with or without clinical diarrhoea. Prev Vet Med. 2011; 100:163-170.
• [16]Stahl M, Kokotovic B, Hjulsager CK, Breum SO, Angen O. The use of quantitative PCR for identification and quantification of Brachyspira pilosicoli, Lawsonia intracellularis and Escherichia coli fimbrial types F4 and F18 in pig feces. Vet Microbiol. 2011; 151:307-314.
• [17]R. R: a language and environment for statistical computing. 3.1.2 ed. Vienna, Austria: R Development Core Team. 2014.
• [18]Moller K, Jensen TK, Jorsal SE, Leser TD, Carstensen B. Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Vet Microbiol. 1998; 62:59-72.
• [19]Cagienard A, Regula G, Danuser J. The impact of different housing systems on health and welfare of grower and finisher pigs in Switzerland. Prev Vet Med. 2005; 68:49-61.
• [20]Stege H, Jensen TK, Moller K, Baekbo P, Jorsal SE. Prevalence of intestinal pathogens in Danish finishing pig herds. Prev Vet Med. 2000; 46:279-292.
• [21]Petersen HH, Nielsen EO, Hassing AG, Ersboll AK, Nielsen JP. Prevalence of clinical signs of disease in Danish finisher pigs. Vet Rec. 2008; 162:377-382.
• [22]Pearce GP. Epidemiology of enteric disease in grower-finisher pigs: a postal survey of pig producers in England. Vet Rec. 1999; 144:338-342.
• [23]Heo JM, Kim JC, Hansen CF, Mullan BP, Hampson DJ, Pluske JR. Feeding a diet with decreased protein content reduces indices of protein fermentation and the incidence of postweaning diarrhea in weaned pigs challenged with an enterotoxigenic strain of Escherichia coli. J Anim Sci. 2009; 87:2833-2843.
• [24]Chase-Topping ME, Gunn G, Strachan WD, Edwards SA, Smith WJ, Hillman K et al.. Epidemiology of porcine non-specific colitis on Scottish farms. Vet J. 2007; 173:353-360.
• [25]Callesen J, Halas D, Thorup F, Knudsen KEB, Kim JC, Mullan BP et al.. The effects of weaning age, diet composition, and categorisation of creep feed intake by piglets on diarrhoea and performance after weaning. Livest Sci. 2007; 108:120-123.
• [26]Funk JA, Davies PR, Gebreyes W. Risk factors associated with Salmonella enterica prevalence in three-site swine production systems in North Carolina, USA. Berl Munch Tierarztl. 2001; 114:335-338.
• [27]Pedersen KS, Skrubel R, Stege H, Angen O, Stahl M, Hjulsager C et al.. Association between average daily gain, faecal dry matter content and concentration of Lawsonia intracellularis in faeces. Acta Vet Scand. 2012; 54:58. BioMed Central Full Text
• [28]Johansen M, Nielsen M, Dahl J, Svensmark B, Baekbo P, Kristensen CS et al.. Investigation of the association of growth rate in grower-finishing pigs with the quantification of Lawsonia intracellularis and porcine circovirus type 2. Prev Vet Med. 2013; 108:63-72.
• [29]Pedersen KS, Stege H, Jensen TK, Guedes R, Stahl M, Nielsen JP et al.. Diagnostic performance of fecal quantitative real-time polymerase chain reaction for detection of Lawsonia intracellularis-associated proliferative enteropathy in nursery pigs. J Vet Diagn Invest. 2013; 25:336-340.
• [30]Collins AM, Barchia IM. The critical threshold of Lawsonia intracellularis in pig faeces that causes reduced average daily weight gains in experimentally challenged pigs. Vet Microbiol. 2014; 168:455-458.