Mycobacteriophage-drived diversification of Mycobacterium abscessus

Biology Direct

Mohamed Sassi¹  Philippe Gouret²  Olivier Chabrol²  Pierre Pontarotti²  Michel Drancourt¹ 

[1] Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes UMR CNRS 6236 IRD198, IFR48, Institut Méditerranée Infection, Aix Marseille Université, Marseille, France

[2] I2M, UMR 7373, EBM 13331Aix Marseille Université, CNRS, Centrale Marseille, Marseille, France

关键词: Mycobacteriophages;    Prophages;    Mycobacterium massiliense;    Mycobacterium bolletii;    Mycobacterium abscessus;   

Others  :  1070619
DOI  :  10.1186/1745-6150-9-19

 received in 2014-03-05, accepted in 2014-08-07,  发布年份 2014

【 摘 要 】

Background

Mycobacterium abscessus is an emerging opportunistic pathogen which diversity was acknowledged by the recent description of two subspecies accommodating M. abscessus, Mycobacterium bolletii and Mycobacterium massiliense isolates.

Results

Here, genome analysis found 1–8 prophage regions in 47/48 M. abscessus genomes ranging from small prophage-like elements to complete prophages. A total of 20,304 viral and phage proteins clustered into 853 orthologous groups. Phylogenomic and phylogenetic analyses based on prophage region homology found three main clusters corresponding to M. abscessus, M. bolletii and M. massiliense. Analysing 135 annotated Tape Measure Proteins found thirteen clusters and four singletons, suggesting that at least 17 mycobacteriophages had infected M. abscessus during its evolution. The evolutionary history of phages differed from that of their mycobacterial hosts. In particular, 33 phage-related proteins have been horizontally transferred within M. abscessus genomes. They comprise of an integrase, specific mycobacteriophage proteins, hypothetical proteins and DNA replication and metabolism proteins. Gene exchanges, loss and gains which occurred in M. abscessus genomes have been driven by several mycobacteriophages.

Conclusions

This analysis of phage-mycobacterium co-evolution suggests that mycobacteriophages are playing a key-role in the on-going diversification of M. abscessus.

Reviewers

This article was reviewed by Eric Bapteste, Patrick Forterre and Eugene Koonin.

【 授权许可】

   
2014 Sassi et al.; licensee BioMed Central Ltd.

【 图 表 】

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