Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy

Biotechnology for Biofuels

Shih-Hsin Ho³  Akihito Nakanishi³  Xiaoting Ye³  Jo-Shu Chang²  Kiyotaka Hara³  Tomohisa Hasunuma³  Akihiko Kondo¹ 

[1] Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seongbuk-gu, Seoul 136-713, Republic of Korea

[2] Center for Bioscience and Biotechnology, National Cheng Kung University, No.1 University Road, Tainan 701, Taiwan

[3] Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan

关键词: Nitrogen depletion;    Salinity-gradient operation;    Metabolite profiling;    Biodiesel;    Lipids;    Chlamydomonas sp;    Microalgae;   

Others  :  791746
DOI  :  10.1186/1754-6834-7-97

 received in 2014-02-17, accepted in 2014-06-06,  发布年份 2014

【 摘 要 】

Background

Biodiesel production from marine microalgae has received much attention as microalgae can be cultivated on non-arable land without the use of potable water, and with the additional benefits of mitigating CO₂ emissions and yielding biomass. However, there is still a lack of effective operational strategies to promote lipid accumulation in marine microalgae, which are suitable for making biodiesel since they are mainly composed of saturated and monounsaturated fatty acids. Moreover, the regulatory mechanisms involved in lipid biosynthesis in microalgae under environmental stress are not well understood.

Results

In this work, the combined effects of salinity and nitrogen depletion stresses on lipid accumulation of a newly isolated marine microalga, Chlamydomonas sp. JSC4, were explored. Metabolic intermediates were profiled over time to observe transient changes during the lipid accumulation triggered by the combination of the two stresses. An innovative cultivation strategy (denoted salinity-gradient operation) was also employed to markedly improve the lipid accumulation and lipid quality of the microalga, which attained an optimal lipid productivity of 223.2 mg L^-1 d^-1 and a lipid content of 59.4% per dry cell weight. This performance is significantly higher than reported in most related studies.

Conclusions

This work demonstrated the synergistic integration of biological and engineering technologies to develop a simple and effective strategy for the enhancement of oil production in marine microalgae.

【 授权许可】

   
2014 Ho et al.; licensee BioMed Central Ltd.

【 图 表 】

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