Synergy of crude enzyme cocktail from cold-adapted Cladosporium cladosporioides Ch2-2 with commercial xylanase achieving high sugars yield at low cost

Biotechnology for Biofuels

Hongli Yuan¹  Ning Zhu¹  Xuejian Yu¹  Baozhen Li¹  Yi Yang¹  Hua Fan¹  Jinshui Yang¹  Lei Ji¹ 

[1]State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China

关键词: Commercial xylanase;    Synergy;    Polysaccharide hydrolases;    MIP;    Laccase;    Cold adaptation;    Cladosporium cladosporioides;   

Others  :  1084540
DOI  :  10.1186/s13068-014-0130-x

 received in 2014-04-28, accepted in 2014-08-22,  发布年份 2014

【 摘 要 】

Background

The efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysaccharide hydrolases and the h ydrolysis of holocellulose. If we include in hydrolase mixture construction the ligninase involved in lignin degradation, which mainly includes laccase, manganese peroxidases (MnP) and lignin peroxidase (LiP), it is feasible that this could greatly improve the fermentable sugars yield.

Results

The psychrophilic lignocellulosic enzymes system of Cladosporium cladosporioides Ch2-2 including ligninase and polysaccharide hydrolases was suitable for selective delignification and efficient saccharification of biomass with wide thermal adaptability. The purified laccase was optimally active at 15°C and pH 3.5, exhibiting high thermostability over a broad range of temperatures (between 4 and 40°C). In addition, manganese-independent peroxidase (MIP), a special type of ligninase with the capacity to oxidize dimethyl phthalate (DMP) in the absence of H₂O₂ and Mn²⁺, was optimally active at 20°C and pH 2.5, exhibiting high thermostability over a broad range of temperatures (4 and 28°C), while depressed completely by Fe²⁺ and essentially unaffected by EDTA. Synergy between Ch2-2 crude enzymes and commercial xylanase obviously enhanced biomass hydrolysis, which could take the place of expensive commercial cellulase mixture. The maximum value of synergistic degree reached 4.7 at 28°C, resulting in 10.1 mg/mL reducing sugars.

Conclusions

The psychrophilic enzymes system of C. cladosporioides Ch2-2 with a different synergistic mechanism has huge potential for the enhancement of biomass hydrolysis at mesophilic and low temperatures. The application scope of the lignocellulosic enzyme cocktail could be greatly enlarged by optimizing the operation conditions specific to the characteristics of ligninase.

【 授权许可】

   
2014 Ji et al.; licensee BioMed Central Ltd.

【 图 表 】

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