Optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture

Biotechnology for Biofuels

Zhicheng Lai¹  Muzi Zhu¹  Xiaofeng Yang¹  Jufang Wang¹  Shuang Li¹ 

[1] Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Panyu District, Guangzhou 510006, China

关键词: Dark fermentation;    Acid hydrolysate;    Sugarcane bagasse;    Non-sterilization;    Thermoanaerobacterium aotearoense SCUT27/Δldh;    Biohydrogen;   

Others  :  1084698
DOI  :  10.1186/s13068-014-0119-5

 received in 2014-02-21, accepted in 2014-07-28,  发布年份 2014

【 摘 要 】

Background

Hydrogen is regarded as an attractive future energy carrier for its high energy content and zero CO₂ emission. Currently, the majority of hydrogen is generated from fossil fuels. However, from an environmental perspective, sustainable hydrogen production from low-cost lignocellulosic biomass should be considered. Thermophilic hydrogen production is attractive, since it can potentially convert a variety of biomass-based substrates into hydrogen at high yields.

Results

Sugarcane bagasse (SCB) was used as the substrate for hydrogen production by Thermoanaerobacterium aotearoense SCUT27/Δldh. The key parameters of acid hydrolysis were studied through the response surface methodology. The hydrogen production was maximized under the conditions of 2.3% of H₂SO₄ for 114.2 min at 115°C. Using these conditions, a best hydrogen yield of 1.86 mol H₂/mol total sugar and a hydrogen production rate (HPR) of 0.52 L/L · h were obtained from 2 L SCB hydrolysates in a 5-L fermentor, showing a superior performance to the results reported in the literature. Additionally, no obvious carbon catabolite repression (CCR) was observed during the fermentation using the multi-sugars as substrates.

Conclusions

Considering these advantages and theimpressive HPR, the potential of hydrogen production using T. aotearoense SCUT27/Δldh is intriguing. Thermophilic, anaerobic fermentation using SCB hydrolysates as the medium by this strain would be a practical and eco-friendly process.

【 授权许可】

   
2014 Lai et al.; licensee Springer

【 图 表 】

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