Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells

Biological Procedures Online

Boon C Heng¹  Catherine M Cowan¹  Shubhayu Basu² 

[1] Abbott Vascular, 3200 Lakeside Dr., Santa Clara, CA 95054, USA

[2] New Business Ventures, Abbott Vascular, 3200 Lakeside Dr., Santa Clara, CA 95054, USA

关键词: Trypsin;    Stem cells;    Mesenchymal;    Enzyme;    Dissociation;   

Others  :  797182
DOI  :  10.1007/s12575-009-9001-4

 received in 2009-01-23, accepted in 2009-01-30,  发布年份 2009

【 摘 要 】

The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequently, the dissociated cells were re-seeded on new cell culture dishes and were subjected to the MTT assay 24 h later. The proportion of viable cells that reattached was significantly lower for cells obtained by dissociation with enzyme-free dissociation buffer compared to trypsin. Frozen–thawed MSC displayed a similar trend, yielding consistently higher cell viability and reattachment rates when dissociated with trypsin compared to enzyme-free dissociation buffer. It was also demonstrated that exposure of trypsin-dissociated MSC to enzyme-free dissociation buffer for 1 h had no significant detrimental effect on cell viability.

【 授权许可】

   
2009 Heng et al.

【 图 表 】

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