Sex differences in the inflammatory response of primary astrocytes to lipopolysaccharide

Biology of Sex Differences

María Santos-Galindo¹  Estefanía Acaz-Fonseca¹  María J Bellini¹  Luis M Garcia-Segura¹ 

[1] Instituto Cajal, CSIC, Avenida Doctor Arce 37, E-28002 Madrid, Spain

关键词: TNFα;    translocator protein 18 kDa;    Toll-like receptor 4;    testosterone;    steroidogenic acute regulatory protein;    IL6;    IL1β;    IFN-inducible protein 10;   

Others  :  793942
DOI  :  10.1186/2042-6410-2-7

 received in 2011-04-04, accepted in 2011-07-11,  发布年份 2011

【 摘 要 】

Background

Numerous neurological and psychiatric disorders show sex differences in incidence, age of onset, symptomatology or outcome. Astrocytes, one of the glial cell types of the brain, show sex differences in number, differentiation and function. Since astrocytes are involved in the response of neural tissue to injury and inflammation, these cells may participate in the generation of sex differences in the response of the brain to pathological insults. To explore this hypothesis, we have examined whether male and female astrocytes show a different response to an inflammatory challenge and whether perinatal testosterone influences this response.

Methods

Cortical astrocyte cultures were prepared from postnatal day 1 (one day after birth) male or female CD1 mice pups. In addition, cortical astrocyte cultures were also prepared from female pups that were injected at birth with 100 μg of testosterone propionate or vehicle. Cultures were treated for 5 hours with medium containing lipopolysaccharide (LPS) or with control medium. The mRNA levels of IL6, interferon-inducible protein 10 (IP10), TNFα, IL1β, Toll-like receptor 4 (TLR4), steroidogenic acute regulatory protein and translocator protein were assessed by quantitative real-time polymerase chain reaction. Statistical significance was assessed by unpaired t-test or by one-way analysis of variance followed by the Tukey post hoc test.

Results

The mRNA levels of IL6, TNFα and IL1β after LPS treatment were significantly higher in astrocytes derived from male or androgenized females compared to astrocytes derived from control or vehicle-injected females. In contrast, IP10 mRNA levels after LPS treatment were higher in astrocytes derived from control or vehicle-injected females than in those obtained from males or androgenized females. The different response of male and female astrocytes to LPS was due neither to differences in the basal expression of the inflammatory molecules nor to differences in the expression of the LPS receptor TLR4. In contrast, the different inflammatory response was associated with increased mRNA levels of translocator protein, a key steroidogenic regulator, in female astrocytes that were treated with LPS.

Conclusions

Male and female cortical astrocytes respond differentially to an inflammatory challenge and this may be predetermined by perinatal testosterone exposure.

【 授权许可】

   
2011 Santos-Galindo et al; licensee BioMed Central Ltd.

【 图 表 】

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