Bacterial diversity of the rock-water interface in an East Antarctic freshwater ecosystem, Lake Tawani(P)†

Aquatic Biosystems

Jonathan P Huang¹  Ashit K Swain²  Robert W Thacker¹  Rasik Ravindra³  Dale T Andersen⁴  Asim K Bej¹ 

[1] Department of Biology, University of Alabama at Birmingham, 1300 University Blvd., CH464, Birmingham, AL 35294-1170, USA

[2] Geological Survey of India, Antarctic Division, NH-5P, NIT, Faridabad 121001, India

[3] Current address: Panikkar Professor and Chairman, INSA-SCAR National Committee, Earth System Science Organization (MoES)Lodi Road, New Delhi 110 003, India

[4] Carl Sagan Center for the Study of Life in the Universe, Mountain View, CA 94043, USA

关键词: Metagenomics;    Pyrosequencing;    bTEFAP;    16S rRNA;    Antarctic freshwater lake;    Culture-dependent;    Culture-independent;   

Others  :  794250
DOI  :  10.1186/2046-9063-9-4

 received in 2012-05-25, accepted in 2013-01-25,  发布年份 2013

【 摘 要 】

Schirmacher Oasis is one of the few ice-free plateaus in East Antarctica that maintains a unique distribution of over 120 microbial-rich, dynamic freshwater lakes, most of which are unexplored. In this study, we describe the bacterial diversity of the rock-water interface in Lake Tawani(P) using culture-independent Bacterial Tag Encoded FLX Amplicon Pyrosequencing (bTEFAP), clone library construction, and culture-based analysis targeting the eubacterial 16S rRNA gene. Lake Tawani(P)was formed in a fossil valley by the accumulation of snow and glacial melt through surface channels into a low-catchment depression. Overall this lake exhibited thirteen bacterial phyla and one-hundred and twelve genera. The Qiime bioinformatics analysis on the bTEFAP alone exhibited higher coverage of the bacterial composition in Lake Tawani(P) than the clone library construction or culture-based methodology. Particularly due to the higher sensitivity of the bTEFAP approach, we detected and differentiated members of the phyla: Chloroflexi, Gemmatimonadetes, Planctomycetes, Nitrospira, and Candidate Division TM7 that other methods were unable to reveal. Nevertheless we found that the use of multiple approaches identified a more complete bacterial community than by using any single approach. Investigating the bacterial diversity of the Schirmacher Oasis lakes, especially those connected through surface channels and encompassed by valleys, will help unravel the dynamic nature of these unique seasonal, freshwater lakes, which potentially harbors highly adapted bacterial taxa with defined ecological functions.

【 授权许可】

   
2013 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

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