Selectively increasing of polyunsaturated (18:2) and monounsaturated (18:1) fatty acids in Jatropha curcas seed oil by crystallization using D-optimal design

Chemistry Central Journal

Jumat Salimon¹  Bashar Mudhaffar Abdullah¹  Nadia Salih¹ 

[1] School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

关键词: Oleic Acid;    Linoleic acid;    Monounsaturated;    Polyunsaturated;    Optimization;    D-optimal design;   

Others  :  788111
DOI  :  10.1186/1752-153X-6-65

 received in 2011-12-09, accepted in 2012-02-10,  发布年份 2012

【 摘 要 】

Background

This study was done to obtain concentrated polyunsaturated fatty acid (PUFA) linoleic acid (LA; 18:2) and monounsaturated fatty acid (MUFA) oleic acid (OA; 18:1) from Jatropha curcas seed oil by urea complexation. Urea complexation is a method used by researchers to separate fatty acids (FAs) based on their molecular structure. Effects the ratio of urea-to-FAs, crystallization temperature and crystallization time on the final products of urea complexation were examined. D-optimal Design was employed to study the significance of these factors and the optimum conditions for the technique were predicted and verified.

Results

Optimum conditions of the experiment to obtain maximum concentration of LA were predicted at urea-to-FAs ratio (w/w) of 5:1, crystallization temperature of −10°C and 24 h of crystallization time. Under these conditions, the final non-urea complex fraction (NUCF) was predicted to contain 92.81% of LA with the NUCF yield of 7.8%. The highest percentage of OA (56.01%) was observed for samples treated with 3:1 urea-to-FAs ratio (w/w) at 10°C for 16 h. The lowest percentage of LA (8.13%) was incorporated into urea complex fraction (UCF) with 1:1 urea-to-FAs ratio (w/w) at 10°C for 8 h.

Conclusions

The separation of PUFA (LA) and MUFA (OA) described here. Experimental variables should be carefully controlled in order to recover a maximum content of PUFA and MUFA of interest with reasonable yield% with a desirable purity of fatty acid of interest.

【 授权许可】

   
2012 Salimon et al.; licensee Chemistry Central Ltd.

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【 参考文献 】

• [1]Hosamani KM, Katagi KS: Characterization and structure elucidation of 12- hydroxyoctadec-cis-9-enoic acid in Jatropha gossypi folia and Hevea brasiliensis seed oils: a rich source of hydroxyl fatty acid. Chem Phys Lipids 2007, 152:9-12.
• [2]Smolinske SC: Handbook of Food, Drug, and Cosmetic Excipients. Michigan, USA: Taylor & Francis, Inc; 1992:247-248.
• [3]Lui S, Zhang C, Hong P, Ji H: Concentration of Docosahexanoic acid (DHA) and eicosapentanoic acid (EPA) of tuna oil by urea complexation: optimizing of process parameters. J Food Eng 2004, 73:203-209.
• [4]Yamamura R, Shimomura Y: Industrial High-Performance Liquid Chromatography Purification of Docosapentaenoic Acid Ethyl Ester from Single-Cell Oil. Ibid 1997, 74:1435-1440.
• [5]Wu M, Ding H, Wang S, Xu Sh: Optimization conditions for the purification of linoleic acid from sunflower oil by urea complex fractionation. J Am Oil Chem Soc 2008, 85:677-684.
• [6]Fie CY, Salimon J, Said M: Optimisation of urea complexation by Box-Behnken design. Sains Malays 2010, 39:795-803.
• [7]Salimon J, Abdullah R: Physicochemical properties of Malaysian Jatropha curcas seed oil. Sains Malays 2008, 37:379-382.
• [8]Guil-Guerrero JL, Belarbi E: Purification process for cod liver oil polyunsaturated fatty acids. J Am Oil Chem Soc 2001, 78:477-484.
• [9]Abu-Nasr AM, Holman RT: Highly unsaturated fatty acids. II. Fractionation by urea inclusion compounds. J Am Oil Chem Soc 1954, 31:16-31.
• [10]Shucheng L, Chaohua Z, Pengzhi H, Hongwu J: Concentration of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) of tuna oil by urea complexation: optimization of process parameters. J Food Eng 2006, 73:203-209.
• [11]Ratnayake WMN, Olsson B, Matthews D, Ackman RG: Preparation of omega-3 PUFA concentrates from fish oils via urea complexation. Lipid 1988, 90:381-386.
• [12]Hayes DG, Bengtsson YC, Van Alstine JM, Setterwall FN: Urea complexation for the rapid, ecologically responsible fractionation of fatty acid from seed oil. J Am Oil Chem Soc 1998, 75:1403-1409.
• [13]Salimon J, Abdullah BM, Salih N: Hydrolysis optimization and characterization study of preparing fatty acids from Jatropha curcas seed oil. Chem Cent J 2011, 5:67. BioMed Central Full Text