Effects on boar semen quality after infection with porcine reproductive and respiratory syndrome virus: a case report

Acta Veterinaria Scandinavica

Martin Schulze³  Sandra Revilla-Fernández²  Friedrich Schmoll²  Rudolf Grossfeld¹  Alfred Griessler⁴ 

[1] Minitüb GmbH, 84184, Tiefenbach, Germany

[2] AGES - Institute for Veterinary Disease Control Mödling, Austrian Agency for Health and Food Safety, 2340, Mödling, Austria

[3] IFN - Institute for Reproduction of Farm Animals Schönow e. V, 16321, Bernau, Germany

[4] Traunkreis Vet Clinic, 4551, Ried im Traunkreis, Austria

关键词: Spermatozoa;    Semen quality;    CASA;    Boar;    PRRSV;   

Others  :  789404
DOI  :  10.1186/1751-0147-55-16

 received in 2012-12-19, accepted in 2013-02-21,  发布年份 2013

【 摘 要 】

The effect of porcine reproductive and respiratory syndrome virus (PRRSV) on semen quality was examined in a group of 11 spontaneously infected boars in a commercial boar stud. Semen samples were collected 4 weeks prior to 4 weeks post-infection (wpi). Infection with PRRSV of the European genotype subtype 1 (EU-1) was verified by specific quantitative real-time polymerase chain reaction (RT-PCR) in 36% of the serum samples. All boars seroconverted before 4 wpi and remained in normal condition throughout the study. Comparison of the percentage of morphologically intact spermatozoa revealed an increase of acrosome-defective spermatozoa (P = 0.012) between −4 and 4 wpi. Significant deleterious effects on semen quality were detected for membrane integrity when semen had been stored for 2 days after sampling. Analysis of sperm subpopulations in a thermoresistance test on day 7 after sampling revealed alterations in the percentage of circular, progressively motile spermatozoa (P = 0.013), in the percentage of non-linear, progressively motile spermatozoa (P = 0.01), and on the amplitude of lateral sperm head displacement (P = 0.047). There was no difference in the incidence of mitochondrially active spermatozoa (P = 0.075). Investigation of routine production data between pre- and post-infection status showed no differences on ejaculate volume (P = 0.417), sperm concentration (P = 0.788), and percentage of motile spermatozoa (P = 0.321). This case report provides insights into a potential control strategy for PRRSV outbreaks in boar studs.

【 授权许可】

   
2013 Schulze et al; licensee BioMed Central Ltd.

【 图 表 】

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