Cell-line-specific stimulation of tumor cell aggressiveness by wound healing factors – a central role for STAT3

BMC Cancer

Lars Ekblad¹  Gustaf Lindgren³  Emma Persson²  Elisabeth Kjellén¹  Johan Wennerberg³ 

[1] Department of Oncology, Lund University, Lund, Sweden

[2] Department of Radiation Sciences, Umeå University, Umeå, Sweden

[3] Department of Otorhinolaryngology/Head and Neck Surgery, Lund University, Lund, Sweden

关键词: Tocilizumab;    IL6R;    IL-6;    STAT3;    Migration;    Invasion;    Proliferation;    Wound healing;    Local recurrence;    Head and neck cancer;   

Others  :  1079957
DOI  :  10.1186/1471-2407-13-33

 received in 2012-09-12, accepted in 2013-01-23,  发布年份 2013

【 摘 要 】

Background

Local recurrence is a major factor affecting survival after treatment for head and neck squamous cell carcinoma (HNSCC). It is possible that the normal processes involved in wound healing after surgical removal of a primary tumor can boost the regrowth of residual cancer cells, thereby contributing to the recurrent growth. In this work, we collected human wound fluids and used them to investigate the effect of wound healing factors on HNSCC cell lines in vitro.

Methods

Wound fluids were collected from thyroidectomized patients diagnosed with benign disease and were included in assays of cell proliferation, migration, cell scattering, and invasion. The involvement of intracellular signaling pathways and membrane receptors were investigated by western blotting and the inclusion of specific inhibitors.

Results

One out of four cell lines was greatly stimulated in proliferation, migration, cell scattering, and invasion by the addition of wound fluid as compared with addition of fetal bovine or human serum. These effects were accompanied by a sharp increase in activation of signal transducer and activator of transcription 3 (STAT3). Inhibition of STAT3 activation abolished the wound fluid response, showing that STAT3 plays an important role in the wound healing response. Several of the observed phenotypic changes were epithelial-to-mesenchymal transition (EMT)-like, but the appropriate changes were not seen in any of the EMT markers investigated. The involvement of c-Met or epidermal growth factor receptor family members was excluded, while the interleukin-6 receptor was found to be partly responsible for the activation of STAT3.

Conclusions

In conclusion, we found cell-line-specific effects of wound healing factors on HNSCC, setting the stage for therapy development and predictive opportunities.

【 授权许可】

   
2013 Ekblad et al; licensee BioMed Central Ltd.

【 图 表 】

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