Ability of vaccine strain induced antibodies to neutralize field isolates of caliciviruses from Swedish cats

Acta Veterinaria Scandinavica

Jonas Johansson Wensman¹  Ayman Samman²  Anna Lindhe⁴  Jean-Christophe Thibault³  Louise Treiberg Berndtsson⁴  Margaret J. Hosie² 

[1] Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden

[2] MRC-University of Glasgow Centre for Virus Research, Glasgow, UK

[3] Global Technical Services, Merial, Lyon, France

[4] Department of Virology, Immune Biology and Parasitology, National Veterinary Institute, Uppsala, Sweden

关键词: Feline upper respiratory tract disease;    Virus neutralization;    Vaccine;    Feline calicivirus;   

Others  :  1234953
DOI  :  10.1186/s13028-015-0178-z

 received in 2015-08-26, accepted in 2015-12-01,  发布年份 2015

【 摘 要 】

Background

Feline calicivirus (FCV) is a common cause of upper respiratory tract disease in cats worldwide. Its characteristically high mutation rate leads to escape from the humoral immune response induced by natural infection and/or vaccination and consequently vaccines are not always effective against field isolates. Thus, there is a need to continuously investigate the ability of FCV vaccine strain-induced antibodies to neutralize field isolates.

Methods

Seventy-eight field isolates of FCV isolated during the years 2008–2012 from Swedish cats displaying clinical signs of upper respiratory tract disease were examined in this study. The field isolates were tested for cross-neutralization using a panel of eight anti-sera raised in four pairs of cats following infection with four vaccine strains (F9, 255, G1 and 431).

Results

The anti-sera raised against F9 and 255 neutralised 20.5 and 11.5 %, and 47.4 and 64.1 % of field isolates tested, respectively. The anti-sera against the more recently introduced vaccine strains G1 and 431 neutralized 33.3 and 70.5 % and 69.2 and 89.7 %, respectively. Dual vaccine strains displayed a higher cross-neutralization.

Conclusions

This study confirms previous observations that more recently introduced vaccine strains induce antibodies with a higher neutralizing capacity compared to vaccine strains that have been used extensively over a long period of time. This study also suggests that dual FCV vaccine strains might neutralize more field isolates compared to single vaccine strains. Vaccine strains should ideally be selected based on updated knowledge on the antigenic properties of field isolates in the local setting, and there is thus a need for continuously studying the evolution of FCV together with the neutralizing capacity of vaccine strain induced antibodies against field isolates at a national and/or regional level.

【 授权许可】

   
2015 Wensman et al.

【 参考文献 】

• [1]Sykes JE. Pediatric feline upper respiratory disease. Vet Clin North Am Small Anim Pract. 2014; 44:331-342.
• [2]Pedersen NC, Elliott JB, Glasgow A, Poland A, Keel K. An isolated epizootic of hemorrhagic-like fever in cats caused by a novel and highly virulent strain of feline calicivirus. Vet Microbiol. 2000; 73:281-300.
• [3]Coyne KP, Jones BR, Kipar A, Chantrey J, Porter CJ, Barber PJ et al.. Lethal outbreak of disease associated with feline calicivirus infection in cats. Vet Rec. 2006; 158:544-550.
• [4]Coyne KP, Edwards D, Radford AD, Cripps P, Jones D, Wood JL et al.. Longitudinal molecular epidemiological analysis of feline calicivirus infection in an animal shelter: a model for investigating calicivirus transmission within high-density, high-turnover populations. J Clin Microbiol. 2007; 45:3239-3244.
• [5]Coyne KP, Christley RM, Pybus OG, Dawson S, Gaskell RM, Radford AD. Large-scale spatial and temporal genetic diversity of feline calicivirus. J Virol. 2012; 86:11356-11367.
• [6]Radford AD, Dawson S, Ryvar R, Coyne K, Johnson DR, Cox MB et al.. High genetic diversity of the immunodominant region of the feline calicivirus capsid gene in endemically infected cat colonies. Virus Genes. 2003; 27:145-155.
• [7]Radford AD, Coyne KP, Dawson S, Porter CJ, Gaskell RM. Feline calicivirus. Vet Res. 2007; 38:319-335.
• [8]Polak KC, Levy JK, Crawford PC, Leutenegger CM, Moriello KA. Infectious diseases in large-scale cat hoarding investigations. Vet J. 2014; 201:189-195.
• [9]Hellard E, Fouchet D, Santin-Janin H, Tarin B, Badol V, Coupier C et al.. When cats’ ways of life interact with their viruses: a study in 15 natural populations of owned and unowned cats (Felis silvestris catus). Prev Vet Med. 2011; 101:250-264.
• [10]Holst BS, Berndtsson LT, Englund L. Isolation of feline herpesvirus-1 and feline calicivirus from healthy cats in Swedish breeding catteries. J Feline Med Surg. 2005; 7:325-331.
• [11]Lauritzen A, Jarrett O, Sabara M. Serological analysis of feline calicivirus isolates from the United States and United Kingdom. Vet Microbiol. 1997; 56:55-63.
• [12]Radford AD, Bennett M, McArdle F, Dawson S, Turner PC, Glenn MA et al.. The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the epidemiological investigation of FCV related disease and vaccine failures. Vaccine. 1997; 15:1451-1458.
• [13]Radford AD, Dawson S, Coyne KP, Porter CJ, Gaskell RM. The challenge for the next generation of feline calicivirus vaccines. Vet Microbiol. 2006; 117:14-18.
• [14]Addie D, Poulet H, Golder MC, McDonald M, Brunet S, Thibault JC et al.. Ability of antibodies to two new caliciviral vaccine strains to neutralise feline calicivirus isolates from the UK. Vet Rec. 2008; 163:355-357.
• [15]Jarrett O, Laird HM, Hay D. Determinants of the host range of feline leukaemia viruses. J Gen Virol. 1973; 20:169-175.
• [16]Poulet H, Jas D, Lemeter C, Coupier C, Brunet S. Efficacy of a bivalent inactivated non-adjuvanted feline calicivirus vaccine: relation between in vitro cross-neutralization and heterologous protection in vivo. Vaccine. 2008; 26:3647-3654.
• [17]Rinaldo D, Foti M, Bottari T, Fisichella V, Buonavoglia D. Feline calicivirus strains isolated in Italy. Pol J Vet Sci. 2008; 11:359-361.
• [18]Radford AD, Turner PC, Bennett M, McArdle F, Dawson S, Glenn MA et al.. Quasispecies evolution of a hypervariable region of the feline calicivirus capsid gene in cell culture and in persistently infected cats. J Gen Virol. 1998; 79:1-10.
• [19]Huang C, Hess J, Gill M, Hustead D. A dual-strain feline calicivirus vaccine stimulates broader cross-neutralization antibodies than a single-strain vaccine and lessens clinical signs in vaccinated cats when challenged with a homologous feline calicivirus strain associated with virulent systemic disease. J Feline Med Surg. 2010; 12:129-137.
• [20]Porter CJ, Radford AD, Gaskell RM, Ryvar R, Coyne KP, Pinchbeck GL et al.. Comparison of the ability of feline calicivirus (FCV) vaccines to neutralise a panel of current UK FCV isolates. J Feline Med Surg. 2008; 10:32-40.
• [21]Poulet H, Brunet S, Soulier M, Leroy V, Goutebroze S, Chappuis G. Comparison between acute oral/respiratory and chronic stomatitis/gingivitis isolates of feline calicivirus: pathogenicity, antigenic profile and cross-neutralisation studies. Arch Virol. 2000; 145:243-261.