Isolation of T cells from mouse oral tissues

Biological Procedures Online

Pushpa Pandiyan¹  Natarajan Bhaskaran¹  Yifan Zhang¹  Aaron Weinberg¹ 

[1] Department of Biological Sciences, School of Dental Medicine, Case Western Reserve University, Cleveland, OH 44106, USA

关键词: ILC;    Th17;    T_reg;    Leukocyte isolation and oral T cells;    Murine oral tissue;   

Others  :  792942
DOI  :  10.1186/1480-9222-16-4

 received in 2013-11-06, accepted in 2014-03-03,  发布年份 2014

【 摘 要 】

Background

Utilizing mouse models provides excellent immunological and experimental tools to study oral immune responses. However for functional assays, isolating T lymphocytes from the oral tissues has proved to be challenging due to the absence of reliable methods that yield viable cells with consistency. To study adaptive immune cell interactions in the oral mucosal tissues, it is necessary to isolate T cells with a good viability and study them at the single cell level.

Findings

We have established an improved method to isolate immune cells, including T_regs and Th17 cells from intra-epithelial niches and lamina propria of the tongue, gingival and palatal tissues in the oral mucosa of mice.

Conclusion

This new method of isolating immune cells from oral tissues will enable us to further our understanding of oral tissue immune cells and their role during oral infections and oral inflammation.

【 授权许可】

   
2014 Pandiyan et al.; licensee BioMed Central Ltd.

【 图 表 】

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