Media composition influences yeast one- and two-hybrid results

Biological Procedures Online

Sarah E Bondos¹  Kathleen S Matthews²  Kim L Gonzalez¹  Hao-Ching Hsiao¹  Zabeena Merchant²  Ying Liu² 

[1]Department of Molecular and Cellular Medicine, Texas A&M University, College Station, TX, 77843 USA

[2]Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005 USA

关键词: false negative;    false positive;    accuracy;    protein-protein interaction;    yeast two-hybrid;    Yeast one-hybrid;   

Others  :  793347
DOI  :  10.1186/1480-9222-13-6

 received in 2011-05-03, accepted in 2011-08-15,  发布年份 2011

【 摘 要 】

Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may optimize signal differences for individual experiments, media preparation must be reported in detail to replicate studies and accurately compare results from different experiments.

【 授权许可】

   
2011 Liu et al; licensee BioMed Central Ltd.

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