Consolidated bioprocessing performance of Thermoanaerobacterium thermosaccharolyticum M18 on fungal pretreated cornstalk for enhanced hydrogen production

Biotechnology for Biofuels

Nan-Qi Ren³  Kun Zhang²  Hong-Yu Ren³  Ai-Jie Wang³  Guang-Li Cao¹  Lei Zhao³ 

[1]School of Life Science and Technology, Harbin Institute of Technology, Harbin 150090, China

[2]College of Power and Energy Engineering, Harbin Engineering University, Harbin 150001, China

[3]State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China

关键词: Cornstalk;    Hydrogen production;    Pretreatment;    Thermoanaerobacterium thermosaccharolyticum M18;    Consolidated bioprocessing;   

Others  :  1088656
DOI  :  10.1186/s13068-014-0178-7

 received in 2014-06-29, accepted in 2014-12-03,  发布年份 2014

【 摘 要 】

Background

Biological hydrogen production from lignocellulosic biomass shows great potential as a promising alternative to conventional hydrogen production methods, such as electrolysis of water and coal gasification. Currently, most researches on biohydrogen production from lignocellulose concentrate on consolidated bioprocessing, which has the advantages of simpler operation and lower cost over processes featuring dedicated cellulase production. However, the recalcitrance of the lignin structure induces a low cellulase activity, making the carbohydrates in the hetero-matrix more unapproachable. Pretreatment of lignocellulosic biomass is consequently an extremely important step in the commercialization of biohydrogen, and for massive realization of lignocellulosic biomass as alternative fuel feedstock. Thus, development of a pretreatment method which is cost efficient, environmentally benign, and highly efficient for enhanced consolidated bioprocessing of lignocellulosic biomass to hydrogen is essential.

Results

In this research, fungal pretreatment was adopted for enhanced hydrogen production by consolidated bioprocessing performance. To confirm the fungal pretreatment efficiency, two typical thermochemical pretreatments were also compared side by side. Results showed that the fungal pretreatment was superior to the other pretreatments in terms of high lignin reduction of up to 35.3% with least holocellulose loss (the value was only 9.5%). Microscopic structure observation combined with Fourier transform infrared spectroscopy (FTIR) analysis further demonstrated that the lignin and crystallinity of lignocellulose were decreased with better holocellulose reservation. Upon fungal pretreatment, the hydrogen yield and hydrogen production rate were 6.8 mmol H₂ g^-1 pretreated substrate and 0.89 mmol L^-1 h^-1, respectively, which were 2.9 and 4 times higher than the values obtained for the untreated sample.

Conclusions

Results revealed that although all pretreatments could contribute to the enhancement of hydrogen production from cornstalk, fungal pretreatment proved to be the optimal method. It is apparent that besides high hydrogen production efficiency, fungal pretreatment also offered several advantages over other pretreatments such as being environmentally benign and energy efficient. This pretreatment method thus has great potential for application in consolidated bioprocessing performance of hydrogen production.

【 授权许可】

   
2014 Zhao et al.; licensee BioMed Central.

【 图 表 】

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【 参考文献 】

• [1]Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS: Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol R 2002, 66:506-577.
• [2]Hasunuma T, Kondo A: Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering. Biotechnol Adv 2012, 30:1207-1218.
• [3]Monlau F, Trably E, Barakat A, Hamelin J, Steyer JP, Carrere H: Two-stage alkaline - enzymatic pretreatments to enhance biohydrogen production from sunflower stalks. Environ Sci Technol 2013, 47:12591-12599.
• [4]Mirza SS, Qazi JI, Zhao Q, Chen S: Photo-biohydrogen production potential of Rhodobacter capsulatus-PK from wheat straw. Biotechnol Biofuels 2013, 6:144. BioMed Central Full Text
• [5]Watanabe T: Introduction: potential of cellulosic ethanol. In Lignocellulose Conversion: Enzymatic and Microbial Tools for Bioethanol Production. Chapter 1. Edited by Faraco V. Springer, Italy; 2013:10-14.
• [6]Cha M, Chung D, Elkins JG, Guss AM, Westpheling J: Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass. Biotechnol Biofuels 2013, 6:85. BioMed Central Full Text
• [7]Lynd LR, van Zyl WH, McBride JE, Laser M: Consolidated bioprocessing of cellulosic biomass: an update. Current Opin Biotechnol 2005, 16:577-583.
• [8]Talluri S, Raj SM, Christopher LP: Consolidated bioprocessing of untreated switchgrass to hydrogen by the extreme thermophile Caldicellulosiruptor saccharolyticus DSM 8903. Bioresour Technol 2013, 139:272-279.
• [9]Olson DG, McBride JE, Shaw AJ, Lynd LR: Recent progress in consolidated bioprocessing. Current Opin Biotechnol 2012, 23:396-405.
• [10]Cao GL, Zhao L, Wang AJ, Wang ZY, Ren NQ: Single-step bioconversion of lignocellulose to hydrogen using novel moderately thermophilic bacteria. Biotechnol Biofuels 2014, 7:82. BioMed Central Full Text
• [11]Agbor VB, Cicek N, Sparling R, Berlin A, Levin DB: Biomass pretreatment: Fundamentals toward application. Biotechnol Adv 2011, 29:675-685.
• [12]Nigam P, Pandey A: Solid-state fermentation technology for bioconversion of biomass and agricultural residues. In Biotechnology for Agro-Industrial Residues Utilisation. Edited by Nigam P, Pandey A. Springer, New York; 2009:197-221.
• [13]Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ: Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 2010, 101:4851-4861.
• [14]Galbe M, Zacchi G: Pretreatment of lignocellulosic materials for efficient bioethanol production. Adv Biochem Eng Biotechnol 2007, 108:41-65.
• [15]Sambusiti C, Monlau F, Ficara E, Carrère H, Malpei F: A comparison of different pre-treatments to increase methane production from two agricultural substrates. Appl Energy 2013, 104:62-70.
• [16]Bak JS, Ko JK, Choi IG, Park YC, Seo JH, Kim KH: Fungal pretreatment of lignocellulose by Phanerochaete chrysosporium to produce ethanol from rice straw. Biotechnol Bioeng 2009, 104:471-482.
• [17]Lo YC, Su YC, Cheng CL, Chang JS: Biohydrogen production from pure and natural lignocellulosic feedstock with chemical pretreatment and bacterial hydrolysis. Int J Hydrogen Energy 2011, 36:13955-13963.
• [18]Zhao L, Cao GL, Wang AJ, Ren HY, Dong D, Liu ZN, Guan XY, Xu CJ, Ren NQ: Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production. Bioresour Technol 2012, 114:365-369.
• [19]Dias AA, Freitas GS, Marques GSM, Sampaio A, Fraga IS, Rodrigues MAM, Evtuguin DV, Bezerra RMF: Enzymatic saccharification of biologically pre-treated wheat straw with white-rot fungi. Bioresour Technol 2010, 101:6045-6050.
• [20]Xu C, Ma F, Zhang X, Chen S: Biological pretreatment of corn stover by Irpex lacteus for enzymatic hydrolysis. J Agric Food Chem 2010, 58:10893-10898.
• [21]Kim Y, Ximenes E, Mosier NS, Ladisch MR: Soluble inhibitors/deactivators of cellulose enzymes from lignocellulosic biomass. Enzyme Microb Technol 2011, 48:408-415.
• [22]Cao WX, Sun C, Liu RH, Yin RZ, Wu XW: Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse. Bioresour Technol 2012, 111:215-221.
• [23]Taherzadeh MJ, Karimi K: Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review. Int J Mol Sci 2008, 9:1621-1651.
• [24]Pandey KK, Pitman AJ: Examination of the lignin content in a softwood and a hardwood decayed by a brown-rot fungus with the acetyl bromide method and Fourier transform infrared spectroscopy. J Polym Sci Pol Chem 2004, 42:2340-2346.
• [25]Yang XW, Zeng YL, Ma FY, Zhang XY, Yu HB: Effect of biopretreatment on thermogravimetric and chemical characteristics of corn stover by different white-rot fungi. Bioresour Technol 2010, 101:5475-5479.
• [26]Bao MD, Su HJ, Tan TW: Dark fermentative bio-hydrogen production: Effects of substrate pre-treatment and addition of metal ions or L-cysteine. Fuel 2013, 112:38-44.
• [27]Gao K, Rehmann L: ABE fermentation from enzymatic hydrolysate of NaOH-pretreated corncobs. Biomass Bioenergy 2014, 66:110-115.
• [28]Zheng MX, Li XJ, Li LQ, Yang XJ, He YF: Enhancing anaerobic biogasification of corn stover through wet state NaOH pretreatment. Bioresour Technol 2009, 100:5140-5145.
• [29]Lin YQ, Wang DH, Wu SQ, Wang CM: Alkali pretreatment enhances biogas production in the anaerobic digestion of pulp and paper sludge. J Hazard Mater 2009, 170:366-373.
• [30]Zhang W, Yi ZL, Huang JF, Li FC, Hao B, Li M, Hong SF, Lv YZ, Sun W, Ragauskas A, Hu F, Peng JH, Peng LC: Three lignocellulose features that distinctively affect biomass enzymatic digestibility under NaOH and H2SO4 pretreatments in Miscanthus. Bioresour Technol 2013, 130:30-37.
• [31]Jung YH, Kim IJ, Kim HK, Kim KH: Dilute acid pretreatment of lignocellulose for whole slurry ethanol fermentation. Bioresour Technol 2013, 132:109-114.
• [32]Zhang QH, Tang L, Zhang JH, Mao ZG, Jiang L: Optimization of thermal-dilute sulfuric acid pretreatment for enhancement of methane production from cassava residues. Bioresour Technol 2011, 102:3958-3965.
• [33]Wan CX, Li YB: Fungal pretreatment of lignocellulosic biomass. Biotechnol Adv 2012, 30:1447-1457.
• [34]Hendriks ATWM, Zeeman G: Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 2009, 100:10-18.
• [35]Wolin EA, Wolin MJ, Wolfe RS: Formation of methane by bacterial extracts. J Biol Chem 1963, 238:2882-2886.
• [36]Hames B: Preparation of Samples for Compositional Analysis. National Renewable Energy Laboratory, Golden, CO, USA; 2005.
• [37]Sluiter A: Determination of Structural Carbohydrates and Lignin in Biomass. National Renewable Energy Laboratory, Golden, CO, USA; 2006.
• [38]Ghose TK: Measurement of cellulase activities. Pure Appl Chem 1987, 59:257-268.
• [39]Zhao L, Cao GL, Wang AJ, Ren HY, Xu CJ, Ren NQ: Enzymatic saccharification of cornstalk by onsite cellulases produced by Trichoderma viride for enhanced biohydrogen production. GCB Bioenergy 2012, 5:591-598.
• [40]Gusakov AV, Salanovich TN, Antonov A, Ustinov BB, Okunev ON, Burlingame R, Emalfarb M, Baez M, Sinitsyn AP: Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose. Biotechnol Biofuels 2007, 97:1028-1038.
• [41]Zhao L, Cao GL, Wang AJ, Guo WQ, Ren HY, Ren NQ: Simultaneous saccharification and fermentation of fungal pretreated cornstalk for hydrogen production using Thermoanaerobacterium thermosaccharolyticum W16. Bioresour Technol 2013, 145:103-107.
• [42]Zhao L, Cao GL, Wang AJ, Ren HY, Ren NQ: Evaluation of continuous biohydrogen production from enzymatically treated cornstalk hydrolysate. Int J Hydrogen Energy 2013, 38:15100-15104.
• [43]Lay JJ, Lee YJ, Noike T: Feasibility of biological hydrogen production from organic fraction of municipal solid waste. Water Res 1999, 33:2579-2586.