Exogenous estradiol improves shell strength in laying hens at the end of the laying period

Acta Veterinaria Scandinavica

Anna Wistedt¹  Yvonne Ridderstråle¹  Helena Wall²  Lena Holm¹ 

[1] Department of Anatomy, Physiology and Biochemistry, SLU, Swedish University of Agricultural Sciences, Box 7011, Uppsala SE-750 07, Sweden

[2] Department of Animal Nutrition and Management, SLU, Swedish University of Agricultural Sciences (Kungsängen Research Center), Uppsala SE-753 23, Sweden

关键词: Domestic hen;    Eggshell quality;    Bone strength;    Estrogen receptors;    Carbonic anhydrase;    Eggshell formation;    Exogenous estradiol;   

Others  :  789197
DOI  :  10.1186/1751-0147-56-34

 received in 2013-09-18, accepted in 2014-05-17,  发布年份 2014

【 摘 要 】

Background

Cracked shells, due to age related reduction of shell quality, are a costly problem for the industry. Parallel to reduced shell quality the skeleton becomes brittle resulting in bone fractures. Calcium, a main prerequisite for both eggshell and bone, is regulated by estrogen in a complex manner. The effects of estrogen, given in a low continuous dose, were studied regarding factors involved in age related changes in shell quality and bone strength of laying hens. A pellet containing 0.385 mg estradiol 3-benzoate (21-day-release) or placebo was inserted subcutaneously in 20 birds each of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) at 70 weeks of age. Eggs were collected before and during the experiment for shell quality measurements. Blood samples for analysis of total calcium were taken three days after the insertion and at sacrifice (72 weeks). Right femur was used for bone strength measurements and tissue samples from duodenum and shell gland were processed for morphology, immunohistochemical localization of estrogen receptors (ERα, ERβ), plasma membrane calcium ATPase (PMCA) and histochemical localization of carbonic anhydrase (CA).

Results

Estrogen treatment increased shell thickness of both hybrids. In addition, shell weight and shell deformation improved in eggs from the brown hybrids. The more pronounced effect on eggs from the brown hybrid may be due to a change in sensitivity to estrogen, especially in surface epithelial cells of the shell gland, shown as an altered ratio between ERα and ERβ. A regulatory effect of estrogen on CA activity, but not PMCA, was seen in both duodenum and shell gland, and a possible connection to shell quality is discussed. Bone strength was unaffected by treatment, but femur was stronger in LSL birds suggesting that the hybrids differ in calcium allocation between shell and bone at the end of the laying period. Plasma calcium concentrations and egg production were unaffected.

Conclusions

A low continuous dose of estrogen improves shell strength but not bone strength in laying hens at the end of the laying period.

【 授权许可】

   
2014 Wistedt et al.; licensee BioMed Central Ltd.

【 图 表 】

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