Molecular typing of Salmonella enterica serovar Enteritidis isolates from food-producing animals in Japan by multilocus variable-number tandem repeat analysis: evidence of clonal dissemination and replacement

Acta Veterinaria Scandinavica

Ayumi Kobayashi⁴  Sayaka Takahashi³  Masaaki Ono³  Kiyoshi Tanaka⁴  Masato Kishima¹  Masato Akiba⁵  Ikuo Uchida² 

[1] Disease Investigation Center Subang, Subang 41212, West Jawa, Indonesia

[2] United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu-shi 501-1193, Japan

[3] Zen-noh Institute of Animal Health, Ojamachi 7, Sakura, Chiba 285-0043, Japan

[4] Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo 062-0045, Hokkaido, Japan

[5] Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka 598-8531, Japan

关键词: Clonal replacement;    MLVA;    Food-producing animals;    Salmonella Enteritidis;   

Others  :  789201
DOI  :  10.1186/1751-0147-56-31

 received in 2013-10-21, accepted in 2014-05-09,  发布年份 2014

【 摘 要 】

Background

Salmonella enterica serovar Enteritidis is a zoonotic pathogen. Human infections are associated with contaminated eggs and egg products. In Japan, since 1989, the incidence of food-borne disease caused by S. Enteritidis has increased and a pandemic has occurred; however, little is known about changes that occurred before and after this pandemic event in the dominant lineage of isolates from food-producing animals. This study aimed to determine the S. Enteritidis lineages in Japan over the last few decades by using multilocus variable-number tandem repeat analysis (MLVA).

Findings

MLVA was used to analyse 79 S. Enteritidis isolates collected from chickens (n = 63), cattle (n = 12), pigs (n = 2), and goats (n = 2) during 1975–2009. The S. Enteritidis isolates showed 14 different MLVA allele combinations, which were classified into two major clusters (A and C) and a minor cluster (B). All the 62 isolates in cluster A were isolated after 1988, whereas 13 of the 17 isolates belonging to cluster B and C were isolated before 1989.

Conclusions

The MLVA results showed that cluster C was predominant before 1989, and isolates in cluster A disseminated since 1989 and replaced the previous dominant clone, suggesting that isolates of cluster A originated from imported S. Enteritidis infection.

【 授权许可】

   
2014 Kobayashi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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