Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study

Basic and Clinical Andrology

Patricia Fauque³  Paul Sagot²  Sophie Girod²  Shaliha Bechoua³  Frédéric Michel¹  Charlène Herbemont³  Céline Bruno³  Laurent Desch³ 

[1]Service de Chirurgie Urologique-Andrologie, Hôpital de Dijon, Université de Bourgogne, Dijon, France

[2]Service de Gynécologie-Obstétrique, Hôpital de Dijon, Université de Bourgogne, Dijon, France

[3]Laboratoire de Biologie de la Reproduction, Hôpital de Dijon, Université de Bourgogne, 2 Bd Mal De Lattre De Tassigny, Dijon, 21079, France

关键词: Testicular sperm;    Outcome;    ICSI;    Freezing;    Culture;   

Others  :  1216968
DOI  :  10.1186/s12610-015-0022-3

 received in 2015-03-31, accepted in 2015-06-01,  发布年份 2015

【 摘 要 】

Purpose

To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1).

Methods

In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantation (IR), pregnancy (PR) and delivery rates (DR)).

Results

The in vitro culture increased total motility (+2.8 %, p = 0.0161) but decreased viability (−8.3 %, p = 0.007). After 24 h of culture, the post-thaw changes in motility and viability were not significant. Twenty-six couples underwent ICSI: thirty–four ICSI were performed with spermatozoa cryopreserved at D0 and eighteen with spermatozoa frozen at D1. Cumulated IR and DR were lower for ICSI with D1 spermatozoa than with D0 spermatozoa (IR: 21.6 % with D0 vs. 9.8 % with D1, p = 0.102; DR: 27.5 % with D0 vs. 8.3 % with D1, p = 0.049).

Conclusion

Despite improving motility, freezing spermatozoa 24 h after testicular biopsy had a potential negative effect on ICSI outcomes, notably on delivery rates. These results may be related to the detrimental impact of the additional culture on the nuclear integrity of sperm.

【 授权许可】

   
2015 Desch et al.

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