Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

Biotechnology for Biofuels

Jesse B Therien²  Oleg A Zadvornyy²  Matthew C Posewitz¹  Donald A Bryant³  John W Peters² 

[1] Department of Chemistry and Geochemistry, Colorado School of Mines, Golden 80401, Colorado, USA

[2] Department of Chemistry and Biochemistry, Montana State University, Bozeman 59717, Montana, USA

[3] Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park 16802, Pennsylvania, USA

关键词: Alginate immobilization;    Co-culture;    Acetate production;    Lipid production;    Cyanobacteria;    Algae;    Biofuels;   

Others  :  1084337
DOI  :  10.1186/s13068-014-0154-2

 received in 2014-06-05, accepted in 2014-10-02,  发布年份 2014

【 摘 要 】

Background

The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC 7002.

Results

Optimal growth conditions for co-cultivation of C. reinhardtii with wild-type and mutant strains of Synechococcus sp. 7002 were established. In co-culture, acetate produced by a glycogen synthase knockout mutant of Synechococcus sp. PCC 7002 was able to support the growth of a lipid-accumulating mutant strain of C. reinhardtii defective in starch production. Encapsulation of Synechococcus sp. PCC 7002 using an alginate matrix was successfully employed in co-cultures to limit growth and maintain the stability.

Conclusions

The ability of immobilized strains of the cyanobacterium Synechococcus sp. PCC 7002 to produce acetate at a level adequate to support the growth of lipid-accumulating strains of C. reinhartdii offers a potentially practical, photosynthetic alternative to providing exogenous acetate into growth media.

【 授权许可】

   
2014 Therien et al.; licensee BioMed Central Ltd.

【 图 表 】

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