Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli

BMC Biotechnology

Yulan Yi²  Huakai Sheng²  Zhimin Li¹  Qin Ye² 

[1] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, Shanghai, China

[2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

关键词: Proline 4-hydroxylases;    Recombinant Escherichia coli;    Recombinant Corynebacterium glutamicum;    Trans-4-hydroxy-L-proline;   

Others  :  834847
DOI  :  10.1186/1472-6750-14-44

 received in 2014-02-07, accepted in 2014-05-15,  发布年份 2014

【 摘 要 】

Background

Trans-4-hydroxy-L-proline (trans-Hyp), one of the hydroxyproline (Hyp) isomers, is a useful chiral building block in the production of many pharmaceuticals. Although there are some natural biosynthetic pathways of trans-Hyp existing in microorganisms, the yield is still too low to be scaled up for industrial applications. Until now the production of trans-Hyp is mainly from the acid hydrolysis of collagen. Due to the increasing environmental concerns on those severe chemical processes and complicated downstream separation, it is essential to explore some environment-friendly processes such as constructing new recombinant strains to develop efficient process for trans-Hyp production.

Result

In this study, the genes of trans-proline 4-hydroxylase (trans-P4H) from diverse resources were cloned and expressed in Corynebacterium glutamicum and Escherichia coli, respectively. The trans-Hyp production by these recombinant strains was investigated. The results showed that all the genes from different resources had been expressed actively. Both the recombinant C. glutamicum and E. coli strains could produce trans-Hyp in the absence of proline and 2-oxoglutarate.

Conclusions

The whole cell microbial systems for trans-Hyp production have been successfully constructed by introducing trans-P4H into C. glutamicum and E. coli. Although the highest yield was obtained in recombinant E. coli, using recombinant C. glutamicum strains to produce trans-Hyp was a new attempt.

【 授权许可】

   
2014 Yi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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