DNA barcoding reveals diversity of Hymenoptera and the dominance of parasitoids in a sub-arctic environment

Annals of Occupational and Environmental Medicine

Julie K Stahlhut³  José Fernández-Triana⁵  Sarah J Adamowicz³  Matthias Buck⁴  Henri Goulet⁵  Paul DN Hebert³  John T Huber⁵  Mark T Merilo³  Cory S Sheffield¹  Thomas Woodcock²  M Alex Smith³ 

[1] Royal Saskatchewan Museum, Regina, SK, Canada

[2] School of Environmental Sciences, University of Guelph, Guelph, ON, Canada

[3] Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada

[4] Royal Alberta Museum, Edmonton, AB, Canada

[5] Canadian National Collection of Insects, Arachnids, and Nematodes, Agriculture and Agri-Food Canada, Ottawa, ON, Canada

关键词: Canada;    Parasitoids;    Sub-Arctic;    Hymenoptera;    DNA barcoding;    Biodiversity;    Barcoding biotas;   

Others  :  1085699
DOI  :  10.1186/1472-6785-13-2

 received in 2012-09-24, accepted in 2012-12-21,  发布年份 2013

【 摘 要 】

Background

Insect diversity typically declines with increasing latitude, but previous studies have shown conflicting latitude-richness gradients for some hymenopteran parasitoids. However, historical estimates of insect diversity and species richness can be difficult to confirm or compare, because they may be based upon dissimilar methods. As a proxy for species identification, we used DNA barcoding to identify molecular operational taxonomic units (MOTUs) for 7870 Hymenoptera specimens collected near Churchill, Manitoba, from 2004 through 2010.

Results

We resolved 1630 MOTUs for this collection, of which 75% (1228) were ichneumonoids (Ichneumonidae + Braconidae) and 91% (1484) were parasitoids. We estimate the total number of Hymenoptera MOTUs in this region at 2624-2840.

Conclusions

The diversity of parasitoids in this sub-Arctic environment implies a high diversity of potential host species throughout the same range. We discuss these results in the contexts of resolving interspecific interactions that may include cryptic species, and developing reproducible methods to estimate and compare species richness across sites and between surveys, especially when morphological specialists are not available to identify every specimen.

【 授权许可】

   
2013 Stahlhut et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Smith MA, Fernandez-Triana J, Roughley R, Hebert PDN: DNA barcode accumulation curves for understudied taxa and areas. Mol Ecol Resour 2009, 9:208-216.
• [2]Fernández-Triana J, Smith MA, Boudreault C, Goulet H, Hebert PDN, Smith AC, Roughley R: A poorly known high-latitude parasitoid wasp community: Unexpected diversity and dramatic changes through time. PLoS One 2011, 6:e23719.
• [3]Zhou X, Adamowicz SJ, Jacobus LM, DeWalt RE, Hebert PD: Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada. Frontiers in Zoology 2009, 6:30. BioMed Central Full Text
• [4]Zhou X, Jacobus LM, DeWalt RE, Adamowicz SJ, Hebert PDN: Ephemeroptera, Plecoptera, and Trichoptera fauna of Churchill (Manitoba, Canada): insights into biodiversity patterns from DNA barcoding. J N Am Benthol Soc 2010, 29:814-837.
• [5]Goulet H, Huber JT: Hymenoptera of the World: An Identification Guide to Families. Ottawa: Research Branch, Agriculture Canada; 1993.
• [6]Hodkinson I, Coulson S: Are high Arctic terrestrial food chains really that simple? - The Bear Island food web revisited. Oikos 2004, 106:427-431.
• [7]Smith MA, Eveleigh ES, McCann KS, Merilo MT, McCarthy PC, Van Rooyen KI: Barcoding a quantified food web: Crypsis, concepts, ecology and hypotheses. PLoS One 2011, 6:e14424.
• [8]Janzen DH: The peak in North American ichneumonid species richness lies between 38 degrees and 42 degrees N. Ecology 1981, 62:532-537.
• [9]Willig MR, Kaufman DM, Stevens RD: Latitudinal gradients of biodiversity: Pattern, process, scale, and synthesis. Annu Rev Ecol Evol Syst 2003, 34:273-309.
• [10]Sime KR, Brower AVZ: Explaining the latitudinal gradient anomaly in ichneumonid species richness: evidence from butterflies. J Anim Ecol 1998, 67:387-399.
• [11]Ricklefs RE: A comprehensive framework for global patterns in biodiversity. Ecol Lett 2004, 7:1-15.
• [12]Skillen EL, Pickering J, Sharkey MJ: Species richness of the Campopleginae and Ichneumoninae (Hymenoptera: Ichneumonidae) along a latitudinal gradient in eastern North American old-growth forests. Environ Entomol 2000, 29:460-466.
• [13]Quicke DLJ: We know too little about parasitoid wasp distributions to draw any conclusions about latitudinal trends in species richness, body size and biology. PLoS One 2012, 7:e32101.
• [14]Gaston KJ, Gauld ID: How many species of pimplines (Hymenoptera: Ichneumonidae) are there in Costa Rica? J Trop Ecol 1993, 9:491-499.
• [15]Smith MA, Rodriguez JJ, Whitfield JB, Deans AR, Janzen DH, Hallwachs W, Hebert PD: Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proc Natl Acad Sci 2008, 105:12359.
• [16]Jones OR, Purvis A, Quicke DLJ: Latitudinal gradients in taxonomic overdescription rate affect macroecological inferences using species list data. Ecography 2012, 35:333-340.
• [17]Strathdee A, Bale J: Life on the edge: Insect ecology in Arctic environments. Annu Rev Entomol 1998, 43:85-106.
• [18]Humble L: Overwintering adaptations in Arctic sawflies (Hymenoptera: Tenthredinidae) and their parasitoids: Cold tolerance. The Canadian Entomologist 2006, 138:59-71.
• [19]Danks HV: Patterns of diversity in the Canadian insect fauna. Memoirs of the Entomological Society of Canada 1993, 125:51-74.
• [20]Fernández-Triana JL: Eight new species and an annotated checklist of Microgastrinae (Hymenoptera, Braconidae) from Canada and Alaska. Zookeys 2010, 63:1-53.
• [21]Danks HV: Seasonal adaptations in Arctic insects. Integr Comp Biol 2004, 44:85-94.
• [22]Valentini A, Pompanon F, Taberlet P: DNA barcoding for ecologists. Trends Ecol Evol 2009, 24:110-117.
• [23]Hebert PDN, Cywinska A, Ball SL, de Waard JR: Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 2003, 270:313-321.
• [24]Blaxter M, Mann J, Chapman T, Thomas F, Whitton C, Floyd R, Abebe E: Defining operational taxonomic units using DNA barcode data. Philosophical Transactions of the Royal Society B: Biological Sciences 2005, 360:1935-1943.
• [25]Schlick-Steiner BC, Steiner FM, Seifert B, Stauffer C, Christian E, Crozier RH: Integrative taxonomy: A multisource approach to exploring biodiversity. Annu Rev Entomol 2010, 55:421-438.
• [26]Gibbs JJ: New species in the Lasioglossum petrellum species group identified through an integrative taxonomic approach. The Canadian Entomologist 2009, 141:371-396.
• [27]Chao A: Nonparametric estimation of the number of classes in a population. Scand J Stat 1984, 11:265-270.
• [28]Chao A: Estimating the population size for capture-recapture data with unequal catchability. Biometrics 1987, 43:783-791.
• [29]Chao A, Hwang W, Chen Y, Kuo C: Estimating the number of shared species in two communities. Stat Sin 2000, 10:227-246.
• [30]Colwell R, Estimate S: Statistical estimation of species richness and shared species from samples. 2009. User’s Guide and application published at: http://purl.oclc.org/estimates webcite
• [31]Chao A, Chazdon RL, Colwell RK, Shen T-J: A new statistical approach for assessing similarity of species composition with incidence and abundance data. Ecol Lett 2005, 8:148-159.
• [32]Fisher BL, Smith MA: A Revision of Malagasy Species of Anochetus Mayr and Odontomachus Latreille (Hymenoptera: Formicidae). PLoS One 2008, 3:e1787.
• [33]Smith MA, Fisher BL: Invasions, DNA barcodes, and rapid biodiversity assessment using ants of Mauritius. Frontiers in Zoology 2009, 6:31. BioMed Central Full Text
• [34]Packer L, Gibbs J, Sheffield C, Hanner R: DNA barcoding and the mediocrity of morphology. Mol Ecol Resour 2009, 9:42-50.
• [35]Dayrat B: Towards integrative taxonomy. Biol J Linn Soc 2005, 85:407-415.
• [36]Huber J: Biodiversity of Hymenoptera. 1st edition. Chichester, UK: Wiley-Blackwell; 2009:303-323. [Insect Biodiversity: Science and Society]
• [37]Gauld ID: Evolutionary patterns of host utilization by ichneumonoid parasitoids (Hymenoptera: Ichneumonidae and Braconidae). Biol J Linn Soc 1988, 35:351-377.
• [38]Wahl D: A review of the mature larvae of Diplazontinae, with notes on larvae of Acaenitinae and Orthocentrinae and proposal of two new subfamilies (Insecta: Hymenoptera, Ichneumonidae). J Nat Hist 1990, 24:27-52.
• [39]Smith MA, Woodley NE, Janzen DH, Hallwachs W, Hebert PD: DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). Proc Natl Acad Sci 2006, 103:3657-3662.
• [40]Smith MA, Wood DM, Janzen DH, Hallwachs W, Hebert PDN: DNA barcodes affirm that 16 species of apparently generalist tropical parasitoid flies (Diptera, Tachinidae) are not all generalists. Proc Natl Acad Sci 2007, 104:4967-4972.
• [41]Kimsey L, Bohart R: Chrysidid Wasps of the World. Oxford: Oxford University Press; 1990.
• [42]Buck M: Identification Atlas of the Vespidae (Hymenoptera, Aculeata) of the northeastern Nearctic region. Canadian Journal of Arthropod Identification 2008.
• [43]Owen DF, Owen J: Species diversity in temperate and tropical Ichneumonidae. Nature 1974, 249:583-584.
• [44]Gauld ID: The Ichneumonidae of Costa Rica, 4: Introduction and keys to species of the subfamilies Metopiinae, Banchinae Atrophini, Banchini, Glyptini. Gainesville, FL: American Entomological Institute; 2002:66.
• [45]Santos AMC, Quicke DLJ: Large‐scale diversity patterns of parasitoid insects. Entomological Science 2011, 14:371-382.
• [46]Gaston K, Gauld I, Hanson P: The size and composition of the hymenopteran fauna of Costa Rica. J Biogeogr 1996, 23:105-113.
• [47]Hespenheide HA: Are there fewer parasitoids in the tropics? Am Nat 1979, 113:766-769.
• [48]Morrison G, Auerbach M, McCoy ED: Anomalous diversity of tropical parasitoids: A general phenomenon? Am Nat 1979, 114:303-307.
• [49]Ivanova NV, Dewaard JR, Hebert PDN: An inexpensive, automation–friendly protocol for recovering high–quality DNA. Molecular Ecology Notes 2006, 6:998-1002.
• [50]Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 1994, 3:294-299.
• [51]Hebert PD, Penton EH, Burns JM, Janzen DH, Hallwachs W: Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci 2004, 101:14812.
• [52]Hajibabaei M, SMITH M, Janzen DH, Rodriguez JJ, Whitfield JB, Hebert PD: A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes 2006, 6:959-964.
• [53]Meusnier I, Singer GA, Landry JF, Hickey DA, Hebert PD, Hajibabaei M: A universal DNA mini-barcode for biodiversity analysis. BMC Genomics 2008, 9:214. BioMed Central Full Text
• [54]Jones M, Ghoorah A, Blaxter M: jMOTU and Taxonerator: Turning DNA barcode sequences into annotated operational taxonomic units. PLoS One 2011, 6:e19259.
• [55]Colwell RK, Chao A, Gotelli NJ, Lin S-Y, Mao CX, Chazdon RL, Longino JT: Models and estimators linking individual-based and sample-based rarefaction, extrapolation and comparison of assemblages. J Plant Ecol 2012, 5:3-21.
• [56]Krebs C, Brzustowski J: Rarefaction Calculator. URL: http://www.biology.ualberta.ca/jbrzusto/rarefact.php webcite. (Undated)