Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT

Biological Procedures Online

J Michael Mathis²  Stephen J Lokitz⁶  Dmitry V Ostanin⁴  Shubnum I Chaudhery³  Elysse A Orchard¹  Anne M Hollister⁵  Shu Yang² 

[1]Department of Pharmacology, Toxicology, and Neurosciences, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA

[2]Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, 1501 Kings Hwy, Shreveport, LA 71130, USA

[3]Department of Pathology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA

[4]Department of Medicine, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA

[5]Department of Orthopedics, Louisiana State University Health Sciences Center, 1501 Kings Hwy, Shreveport, LA 71130, USA

[6]PET Imaging Center, Biomedical Research Foundation of Northwest Louisiana, Shreveport, LA 71130, USA

关键词: Disease index;    Bone volume;    Bone density;    Collagen induced arthritis;    Imaging;    Computed tomography;    Micro-CT;    Rheumatoid arthritis;   

Others  :  793061
DOI  :  10.1186/1480-9222-15-8

 received in 2013-05-27, accepted in 2013-06-29,  发布年份 2013

【 摘 要 】

Background

Inflammatory arthritis is a chronic disease, resulting in synovitis and subchondral and bone area destruction, which can severely affect a patient’s quality of life. The most common form of inflammatory arthritis is rheumatoid arthritis (RA) in which many of the disease mechanisms are not well understood. The collagen-induced arthritis (CIA) mouse model is similar to RA as it exhibits joint space narrowing and bone erosion as well as involves inflammatory factors and cellular players that have been implicated in RA pathogenesis. Quantitative data for disease progression in RA models is difficult to obtain as serum blood markers may not always reflect disease state and physical disease indexes are subjective. Thus, it is important to develop tools to objectively assess disease progression in CIA.

Results

Micro-CT (Computed Tomography) is a relatively mature technology that has been used to track a variety of anatomical changes in small animals. In this study, micro-CT scans of several joints of control and CIA mice were acquired at 0, 4, 7, and 9 weeks after the immunization with collagen type II. Each micro-CT scan was analyzed by applying a segmentation algorithm to individual slices in each image set to provide 3-dimensional representations of specific bones including the humerus, femur, and tibia. From these representations, the volume and mean density of these bones were measured and compared. This analysis showed that both the volume and the density of each measured bone of the CIA mice were significantly smaller than those of the controls at week 7.

Conclusions

This study demonstrates that micro-CT can be used to quantify bone changes in the CIA mouse model as an alternative to disease index assessments. In conclusion, micro-CT could be useful as a non-invasive method to monitor the efficacy of new treatments for RA tested in small animals.

【 授权许可】

   
2013 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

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