Studies on bioflocculant production by a mixed culture of Methylobacterium sp. Obi and Actinobacterium sp. Mayor

BMC Biotechnology

Ntsaluba Luvuyo¹  Uchechukwu U Nwodo¹  Leonard V Mabinya¹  Anthony I Okoh¹ 

[1] Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

关键词: Functional groups;    Thermostable;    Flocculating activity;    Consortium;    Bioflocculant;   

Others  :  1121358
DOI  :  10.1186/1472-6750-13-62

 received in 2013-05-03, accepted in 2013-07-25,  发布年份 2013

【 摘 要 】

Background

Bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. Consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures.

Results

16S rDNA nucleotide sequencing and BLAST analysis of nucleotide sequences were used to identify the bacterial species, carbon and nitrogen sources optimally supporting bioflocculant production were assessed and the purified bioflocculant characterized.

Nucleotide sequences showed 97% and 96% similarity to Methylobacterium sp. AKB-2008-KU9 and Methylobacterium sp. strain 440. The second isolate, likewise, showed 98% similarity to Actinobacterium OR-221. The sequences were deposited in GenBank as Methylobacterium sp. Obi [accession number HQ537130] and Actinobacterium sp. Mayor [accession number JF799090]. Flocculating activity of 95% was obtained in the presence of Ca²⁺ and heat-stability was exhibited with retention of above 70% activity at 100°C in 30 min. In addition, bioflocculant yield was about 8.203 g/l. A dose of 1 mg/ml of purified bioflocculant was optimal for the clarification of Kaolin suspension (100 ml) following Jar test. FTIR spectrum revealed the presence of carboxyl and hydroxyl functional groups amongst others.

Conclusions

The mixed culture produced bioflocculant with high flocculating activity and an improved yield. The efficiency observed with jar test may imply industrial applicability.

【 授权许可】

   
2013 Luvuyo et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Koizumi JI, Takeda M, Kurane R, Nakamura I: Synergetic flocculating of the bioflocculants FIX extracellularly produced by Nocardiaamare. J Gen Appl Microbiol 1991, 37:447-457.
• [2]Sanayei Y, Ismail N, Teng TT, Morad N: Studies on flocculating activity of bioflocculant from closed drainage system (CDS) and its application in reactive dye removal. International J Chem 2010, 2(1):168-173.
• [3]Kurane R, Matsuyama H: Production of a bioflocculant by mixed culture. Biosci Biotechnol Biochem 1994, 58:1589-1594.
• [4]Patil SV, Salunkhe RB, Patil CD, Patil DM: Bioflocculant exopolysaccharide production by Azotobacterinducus using flower extract of Madhucalatifolia L. Appl Biochem Biotechnol 2011, 162:1095-1108.
• [5]Matthys C, Bilau M, Govaert Y, Moons E, De HS, Willems JL: Risk assessment of dietary acrylamide intake in Flemish adolescents. Food Chem Toxicol 2005, 43:271-278.
• [6]He N, Li Y, Chen J: Production of a polygalacturonic acid bioflocculant REA-11 by Corynebacterium glutamicum. Bioresour Technol 2004, 94:99-105.
• [7]Salehizadeh H, Shojaosadati SA: Extracellular biopolymeric flocculants recent trends and biotechnological importance. Biotechnol Adv 2011, 19:371-385.
• [8]Kurane R, Hatamochi K, Kakuno T, Kiyohara M, Hirono M, Taniguchi T: Production of a bioflocculant by Rhodococcus erythropolis S-1 grown on alcohols. Biosci Biotechnol Biochem 1994, 58:428-429.
• [9]Li Y, He N, Guan H, Du G, Chen J: A polygalacturonic acid bioflocculant REA-11 produced by Corynebacterium glutamicum: a proposed biosynthetic pathway and experimental confirmation. Appl Microbiol Biotechnol 2003, 63:200-206.
• [10]He J, Zou J, Shao Z, Zhang J, Liu Z, Yu Z: Characteristics flocculating mechanism of a novel bioflocculant HBF-3 produced by deep-sea bacterium mutant Halomonas sp. V3a. J Microbiol Biotechnol 2010, 26:1135-1141.
• [11]Xia S, Zhang Z, Wang X, Yang A, Chen L, Zhao J, Leonard D, Jaffrezic-Renault N: Production and characterization of bioflocculant by Proteus mirabilis TJ-1. Bioresour Technol 2008, 99:6520-6527.
• [12]Ma F, Liu JL, Li SG, Yang JX, Zhang LQ, Wu B, Zhu YB: Development of complex microbial flocculant. China Water Wastewater 2003, 19:1-4.
• [13]Zhang ZQ, Lin B, Xia SQ, Wang XJ, Yang AM: Production and application of a novel bioflocculant by multiple microorganism consortia using brewery wastewater as carbon source. J Environ Sci 2007, 19:667-673.
• [14]Wang Y, Gao BY, Yue QY, Wei JC, Zhou WZ, Gu R: Color removal from textile industry wastewater using composite flocculants. Environ Technol 2010, 28(6):629-637.
• [15]Chang WC, Soon AY, In HO, Sang HP: Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp. Biotechnol Lett 1998, 20(12):643-646.
• [16]Jie G, Hua-ying B, Ming-xia X, Qian L, Yan-fen : Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31. J Zhejiang Univ Sci B 2006, 7(3):186-192.
• [17]Wang S, Gong W, Lui X, Tian L, Yue Q, Gao B: Production of novel bioflocculant by culture of Klebsiella mobilis using dairy wastewater. J Biochem Eng 2007, 39:81-86.
• [18]Nwodo UU, Agunbiade MO, Green E, Mabinya LV, Okoh AI: A Freshwater Streptomyces, isolated from Tyume River, produces a predominantly extracellular glycoprotein bioflocculant. Int J Mol Sci 2012, 13:8679-8695.
• [19]Chan WC, Chiang CY: Flocculation of clay suspensions with water insoluble starch grafting acrylamide/sodium allylsulfonated copolymer powder. Appl Polymer Sci 1995, 58:1721-1726.
• [20]Wang L, Ma F, Qu Y, Sun D, Li A, Guo J, Guo J, Yu B: Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6. World J Microbiol Biotechnol 2011, 10:1007-1012.
• [21]Zheng Y, Ye Z, Fang X, Li Y, Cia W: Production and characteristics of a bioflocculant produced by Bacillus sp. F19. Biosour Technol 2008, 99:7686-7691.
• [22]Liu WJ, Wang K, Li BZ, Yuan HL, Yang JS: Production and characterization of an intracellular bioflocculant by Chryseobacterium daeguense W6 cultured in low nutrition medium. Bioresour Technol 2010, 101:1044-1048.
• [23]Zhi L, Baoping H, Hong L: Optimum condition to high-concentration microparticle slime water with bioflocculants. Mining Sci Technol 2010, 20:0478-0484.
• [24]Suh H, Kwon G, Lee C, Kim H, Yoon B: Characterization of bioflocculant produced by Bacillus sp. DP-152. J Ferment Bioeng 1997, 84:108-112.
• [25]Kumar CG, Joo HS, Kavali R, Choi JW, Chang CS: Characterization of an extracellular biopolymer flocculant from a haloakalophilic Bacillus isolate. World J Microbiol Biotechnol 2004, 20(8):837-843.
• [26]Comte S, Guibaud G, Baudu M: Bio sorption properties of extracellular polymeric substances (EPS) resulting from activated sludge according to their type: soluble or bound. Proces Biochem 2006, 41:815-823.
• [27]Kumar CG, Anand SK: Significance of microbial biofilms in food industry: a review. Int J Food Microbiol 1998, 42:9-27.