Keratinocyte cytoskeletal roles in cell sheet engineering

BMC Biotechnology

Qi Wei¹  Daniel Reidler¹  Min Ye Shen¹  Hayden Huang¹ 

[1] Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 500 W 120th Street, MC 8904, New York, NY 10027, USA

关键词: Contraction;    Adhesion;    Cytoskeleton;    Cell sheet;   

Others  :  1123241
DOI  :  10.1186/1472-6750-13-17

 received in 2012-08-23, accepted in 2013-02-22,  发布年份 2013

【 摘 要 】

Background

There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragile and, over time, will contract. While the role of the cytoskeleton in maintaining the structure and adhesion of cells cultured on a rigid substrate is well-characterized, a systematic examination of the role played by different components of the cytoskeleton in regulating cell sheet contraction and cohesion in the absence of a substrate has been lacking.

Results

In this study, keratinocytes were cultured until confluent and cell sheets were generated using dispase to remove the influence of the substrate. The effects of disrupting actin, microtubules or intermediate filaments on cell-cell interactions were assessed by measuring cell sheet cohesion and contraction. Keratin intermediate filament disruption caused comparable effects on cell sheet cohesion and contraction, when compared to actin or microtubule disruption. Interfering with actomyosin contraction demonstrated that interfering with cell contraction can also diminish cell cohesion.

Conclusions

All components of the cytoskeleton are involved in maintaining cell sheet cohesion and contraction, although not to the same extent. These findings demonstrate that substrate-free cell sheet biomechanical properties are dependent on the integrity of the cytoskeleton network.

【 授权许可】

   
2013 Wei et al; licensee BioMed Central Ltd.

【 图 表 】

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