Kinetics of arsenite removal by halobacteria from a highland Andean Chilean Salar

Aquatic Biosystems

Contreras-Ortega Carlos¹  Gallardo Karem¹  Arias Diana¹  Silva Pedro¹  Rojo Gonzalo¹  Queirolo Fabrizio¹  Stegen Susana¹  Pozo Patricia¹  Hengst Martha³  Alfaro Gleny¹  Díaz-Palma Paula² 

[1]Departamento de Química, Facultad de Ciencias, Universidad Católica del Norte, P.O.1280, Antofagasta, Chile

[2]Advanced Mining Technology Center, AMTC, Universidad de Chile, Tupper 2007, Santiago, Chile

[3]Laboratorio de Complejidad Microbiana y Ecología Funcional, Facultad de Recursos del Mar & Centro de BioInnovación de Antofagasta, Universidad de Antofagasta, Antofagasta, Chile

关键词: Arsenite-Oxidase;    Halobacteria;    Removal;    Arsenic;   

Others  :  794199
DOI  :  10.1186/2046-9063-9-8

 received in 2012-07-17, accepted in 2013-02-21,  发布年份 2013

【 摘 要 】

Background

The purpose of this study was to identify arsenite-oxidizing halobacteria in samples obtained from Salar de Punta Negra, II Region of Chile. Seven bacterial isolates, numbered as isolates I to VII, grown in a culture medium with 100 ppm as NaAsO₂ (As (III)) were tested. Bacterial growth kinetics and the percent of arsenite removal (PAR) were performed simultaneously with the detection of an arsenite oxidase enzyme through Dot Blot analysis.

Results

An arsenite oxidase enzyme was detected in all isolates, expressed constitutively after 10 generations grown in the absence of As (III). Bacterial growth kinetics and corresponding PAR values showed significant fluctuations over time. PARs close to 100% were shown by isolates V, VI, and VII, at different times of the bacterial growth phase; while isolate II showed PAR values around 40%, remaining constant over time.

Conclusion

Halobacteria from Salar de Punta Negra showed promising properties as arsenite removers under control conditions, incubation time being a critical parameter.

【 授权许可】

   
2013 Paula et al.; licensee BioMed Central Ltd.

【 图 表 】

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