Treatment of rice straw hemicellulosic hydrolysates with advanced oxidative processes: a new and promising detoxification method to improve the bioconversion process

Biotechnology for Biofuels

João Paulo Alves Silva¹  Livia Melo Carneiro¹  Inês Conceição Roberto¹ 

[1] Deparatmento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho s/n, Cep, 12602-810, Lorena, SP, Brazil

关键词: Detoxification;    Advanced oxidative processes;    Rice straw hemicellulosic hydrolysate;    Pichia stipitis;    Ethanol;   

Others  :  798150
DOI  :  10.1186/1754-6834-6-23

 received in 2012-09-28, accepted in 2013-01-31,  发布年份 2013

【 摘 要 】

Background

The use of lignocellulosic constituents in biotechnological processes requires a selective separation of the main fractions (cellulose, hemicellulose and lignin). During diluted acid hydrolysis for hemicellulose extraction, several toxic compounds are formed by the degradation of sugars and lignin, which have ability to inhibit microbial metabolism. Thus, the use of a detoxification step represents an important aspect to be considered for the improvement of fermentation processes from hydrolysates. In this paper, we evaluated the application of Advanced Oxidative Processes (AOPs) for the detoxification of rice straw hemicellulosic hydrolysate with the goal of improving ethanol bioproduction by Pichia stipitis yeast. Aiming to reduce the toxicity of the hemicellulosic hydrolysate, different treatment conditions were analyzed. The treatments were carried out according to a Taguchi L₁₆ orthogonal array to evaluate the influence of Fe⁺², H₂O₂, UV, O₃ and pH on the concentration of aromatic compounds and the fermentative process.

Results

The results showed that the AOPs were able to remove aromatic compounds (furan and phenolic compounds derived from lignin) without affecting the sugar concentration in the hydrolysate. Ozonation in alkaline medium (pH 8) in the presence of H₂O₂ (treatment A3) or UV radiation (treatment A5) were the most effective for hydrolysate detoxification and had a positive effect on increasing the yeast fermentability of rice straw hemicellulose hydrolysate. Under these conditions, the higher removal of total phenols (above 40%), low molecular weight phenolic compounds (above 95%) and furans (above 52%) were observed. In addition, the ethanol volumetric productivity by P. stipitis was increased in approximately twice in relation the untreated hydrolysate.

Conclusion

These results demonstrate that AOPs are a promising methods to reduce toxicity and improve the fermentability of lignocellulosic hydrolysates.

【 授权许可】

   
2013 Silva et al.; licensee BioMed Central Ltd.

【 图 表 】

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