The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest

Biotechnology for Biofuels

Cécile Neuvéglise^1,10  Uwe Scholz^1,11  Patrick Wincker^1,16  Jean-Luc Souciet⁸  Bernard Dujon^1,15  Sebastian Worch^1,11  Eric Westhof⁹  Benoit Vacherie⁷  Anke Trautwein-Schult^1,11  Agnès Thierry^1,15  Marie-Laure Straub⁸  Nils Stein^1,11  David J Sherman^2,20  Joseph Schacherer⁸  Guilhem Savel^2,20  Anasua Sarkar^2,20  Christine Sacerdot⁶  Jan Riechen^1,11  Guy-Franck Richard^1,15  Guillaume Morel^1,10  Martin Mascher^1,11  Marina Marcet-Houben^2,21  Marc Lemaire^1,12  Véronique Leh-Louis⁸  Ingrid Lafontaine^1,15  Paul Jung⁸  Claire Jubin^1,16  Dagmara Jankowska^1,11  Anja Hartmann^1,11  Urs Hähnel^1,11  Przemysław Piotr Gierski^1,17  Martin Giersberg^1,11  Cécile Fairhead¹  Laurence Despons⁸  Serge Casaregola^1,10  Rüdiger Bode^1,18  Claudine Bleykasten⁸  Sebastian Beier^1,11  Keith Baronian^1,14  Philippe Baret^1,19  Valérie Barbe⁷  André Goffeau⁴  Christian Marck²  Emmanuel Talla³  Jose A Cruz⁹  Toni Gabaldón^2,21  Erik Böer^1,11  Tiphaine Martin^2,20  Pascal Durrens^2,20  Małgorzata Czernicka⁵  Claude Gaillardin^1,10  Gotthard Kunze^1,13 

[1]Institut de Génétique et Microbiologie, Université Paris-Sud, UMR CNRS 8621, F- Orsay CEDEX 91405, France

[2]CEA, Saclay Biology and Technologies Institute (iBiTec-S), Gif-sur-Yvette F-91191, France

[3]Aix-Marseille Université, CNRS UMR 7283, Laboratoire de Chimie Bactérienne, F-13402 Marseille, Cedex 20, France

[4]Université catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 5/15, Louvain-la-Neuve 1349, Belgium

[5]Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, Al. 29 Listopada 54, Krakow 31-425, Poland

[6]Present address: École Normale Supérieure, Institut de Biologie de l’ENS (IBENS), 46 rue d’Ulm, Paris F-75005, France

[7]CEA, Institut de Génomique, Genoscope, 2 Rue Gaston Crémieux, Évry F-91000, France

[8]Université de Strasbourg, CNRS UMR7156, Strasbourg F-67000, France

[9]Université de Strasbourg, Architecture et Réactivité de l’ARN, Institut de Biologie Moléculaire et Cellulaire du CNRS, F-67084 Strasbourg, France

[10]INRA French National Institute for Agricultural Research, Micalis UMR 1319, CBAI, Thiverval-Grignon F-78850, France

[11]Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben D-06466, Germany

[12]Université Lyon 1, CNRS UMR 5240, Villeurbanne F-69621, France

[13]Yeast Genetics, Leibniz Institute of Plant Research (IPK), Corrensstrasse 3, Gatersleben 06466, Germany

[14]School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand

[15]Institut Pasteur, Université Pierre et Marie Curie UFR927, CNRS UMR 3525, F-75724 Paris-CEDEX 15, France

[16]Université d’Evry, Bd François Mitterand, Evry F-91025, France

[17]Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology in Warsaw, ul. Ks. Trojdena 4, Warsaw 02-109, Poland

[18]Institute of Biochemistry, University of Greifswald, Felix-Hausdorffstraße 4, Greifswald D-17487, Germany

[19]Université Catholique de Louvain, Earth and Life Institute (ELI), Louvain-la-Neuve 1348, Belgium

[20]LaBRI (UMR 5800 CNRS) and project-team Magnome INRIA Bordeaux Sud-Ouest, Talence F-33405, France

[21]Universitat Pompeu Fabra (UPF), Barcelona 08003, Spain

关键词: Metabolism;    n-butanol;    Tannic acid;    Biotechnology;    Genome;    Yeast;   

Others  :  792547
DOI  :  10.1186/1754-6834-7-66

 received in 2013-11-13, accepted in 2014-03-19,  发布年份 2014

【 摘 要 】

Background

The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant.

Results

The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid.

Conclusions

The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.

【 授权许可】

   
2014 Kunze et al.; licensee BioMed Central Ltd.

【 图 表 】

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