Bioethanol production from rice straw by popping pretreatment

Biotechnology for Biofuels

Seung Gon Wi²  In Seong Choi³  Kyoung Hyoun Kim¹  Ho Myeong Kim¹  Hyeun-Jong Bae¹ 

[1] Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea

[2] Bio-energy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea

[3] Department of Forest Products and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea

关键词: Fermentation;    Enzymatic hydrolysis;    Bioethanol;    Rice straw;    Popping pretreatment;   

Others  :  794596
DOI  :  10.1186/1754-6834-6-166

 received in 2013-05-29, accepted in 2013-09-24,  发布年份 2013

【 摘 要 】

Background

Rice straw has considerable potential as a raw material for bioethanol production. Popping pretreatment of rice straw prior to downstream enzymatic hydrolysis and fermentation was found to increase cellulose to glucose conversion efficiency. The aim of this study was to investigate the influence of popping pretreatment and determine the optimal enzyme loading using a surface response design.

Results

The optimal doses of cellulase and xylanase enzymes were 23 FPU and 62 IU/g biomass, respectively. Using the optimized enzyme condition and popping pretreatment of rice straw (15% substrate loading, w/v), a sugar recovery of 0.567 g/g biomass (glucose; 0.394 g/g) was obtained in 48 h, which was significantly higher than that from untreated rice straw (total sugar recovery; 0.270 g/g biomass). Fermentation of the hydrolyzates by Saccharomyces cerevisiae resulted in 0.172 g ethanol/g biomass after 24 h, equivalent to 80.9% of the maximum theoretical yield (based on the amount of glucose in raw material). Changes in the chemical composition and surface area of rice straw were also investigated before and after popping pretreatment. The results showed little or no difference in chemical composition between the pretreated rice straw and the control. However, the surface area of pretreated rice straw increased twofold compared to the control.

Conclusion

Popping pretreatment of rice straw can effectively improve downstream saccharification and fermentation, important for bioethanol production.

【 授权许可】

   
2013 Wi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Kim S, Dale BE: Global potential bioethanol from wasted crops and crop residues. Biomass Bioenerg 2004, 26:361-375.
• [2]Naik SN, Goud VV, Rout PK, Dalai AK: Production of first and second generation biofuels: a comprehensive review. Renew Sust Energ Rev 2010, 14:578-597.
• [3]Sarkar N, Ghosh SK, Bannerjee S, Aikat K: Bioethanol production from agricultural wastes: an overview. Renew Energ 2012, 37:19-27.
• [4]McAloon A, Taylor F, Yee W, Ibsen K, Wooley R: Determining the cost of producing ethanol from corn starch and lignocellulosic feedstocks. NREL/TP-580-28893. Golden, CO (USA): National Renewable Energy Laboratory; 2000.
• [5]Cherubini F, Ulgiati S: Crop residues as raw materials for biorefinery systems – A LCA case study. Appl Energy 2010, 87:47-57.
• [6]Galbe M, Zacchi G: A review of the production of ethanol from softwood. Appl Microbiol Biotechnol 2002, 59:618-628.
• [7]Wyman CE, Hinman ND: Ethanol - Fundamentals of production from renewable feedstocks and use a transportation fuel. Appl Biochem Biotechnol 1990, 24/25:735-753.
• [8]Balat M, Balat H, Öz C: Progress in bioethanol processing. Prog Energy Combust Sci 2008, 4:551-573.
• [9]Binod P, Sindhu R, Singhania RR, Vikram S, Devi L, Nagalakshmi S, Kurien N, Sukumaran RK, Pandey A: Bioethanol production from rice straw: an overview. Bioresour Technol 2010, 101:4767-4774.
• [10]Hu G, Heitmann JA, Rojas OJ: Feedstock pretreatment strategies. BioResources 2008, 3:270-294.
• [11]Mosier N, Wyman H, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M: Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol 2005, 96:673-686.
• [12]Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ: Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review. Bioresour Technol 2010, 101:4851-4861.
• [13]Li Q, He YC, Xian M, Jun G, Xu X, Yang JM, Li LZ: Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment. Bioresour Technol 2009, 100:3570-3575.
• [14]Wi SG, Chung BY, Lee YG, Yang DJ, Bae H-J: Enhanced enzymatic hydrolysis of rapeseed straw by popping pretreatment for bioethanol production. Bioresour Technol 2011, 102:5788-5793.
• [15]Choi IS, Kim J-H, Wi SG, Kim KH, Bae H-J: Bioethanol production from mandarin (Citrus unshiu) peel waste using popping pretreatment. Appl Energy 2012, 102:204-210.
• [16]Choi IS, Wi SG, Kim S-B, Bae H-J: Conversion of coffee residue waste into bioethanol with using popping pretreatment. Bioresour Technol 2012, 125:132-137.
• [17]Lv S, Yu Q, Zhuang X, Yuan Z, Wang W, Wang Q, Qi W, Tan X: The Influence of hemicellulose and lignin removal on the enzymatic digestibility from sugarcane bagasse. Bioenerg Res 2013. DOI 10.1007/s12155-013-9297-4
• [18]Zhao X, Cheng K, Liu D: Organosolv pretreatment of lignocellosic biomass for enzymatic hydrolysis. Appl Microbial Biotechnol 2009, 82:815-827.
• [19]Tabka MG, Herpoel-Gimbert I, Monod F, Asther M, Sigoillot JC: Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase xylanase and feruloyl esterase treatment. Enzyme Microb Tech 2006, 39:897-902.
• [20]Romaní A, Garrote G, Parajó JC: Bioethanol production from autohydrolyzed Eucalyptus globulus by Simultaneous Saccharification and Fermentation operating at high solids loading. Fuel 2012, 94:305-312.
• [21]Palmqvist E, Hahn-Hägerdal B: Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification. Bioresour Technol 2000, 74:17-24.
• [22]Abedinifar S, Karimi K, Khanahmadi M, Taherzadeh MJ: Ethanol production by Mucor indicus and Rhizopus oryzae from rice straw by separate hydrolysis and fermentation. Bioresour Technol 2009, 33:828-833.
• [23]Chen W-H, Lin T-S, Guo G-L, Huang W-S: Ethanol production form rice straw hydrolysates by Pichia stipitis. Energy Procedia 2012, 14:1261-1266.
• [24]Karimi K, Emtiazi G, Taherzadeh MJ: Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme Microbial Technol 2006, 40:138-144.
• [25]Ko JK, Bak JS, Jung MW, Lee HJ, Choi IG, Kim TH, et al.: Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes. Bioresour Technol 2009, 100:4374-4380.
• [26]TAPPI Committee: TAPPI test methods. Atlanta, GA (USA): TAPPI Press; 1992.
• [27]Adney B, Baker J: Measurement of cellulase activities. NREL/TP-510-42628. Golden, CO (USA): National Renewable Energy Laboratory; 2008.
• [28]Teixeira RSS, Siqueira FG, Souza MVD, Filho EXF, Bon EPDS: Purification and characterization studies of a thermostable β-xylanase from Aspergillus awamori. J Ind Microbiol Biotechnol 2010, 37:1041-1051.
• [29]Miller GL: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959, 13:426-428.
• [30]Box GEP, Wilson KG: On the experimental attainment of optimum conditions. J R Stat Soc Ser B-Stat Methodol 1951, 13:1-45.