Comparative quantitative proteomics of prochlorococcus ecotypes to a decrease in environmental phosphate concentrations

Aquatic Biosystems

Matthew A Fuszard¹  Phillip C Wright¹  Catherine A Biggs¹ 

[1] ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK

关键词: Phosphate;    Growth;    PhoE;    PhoA;    PstS;    Prochlorococcus;   

Others  :  794721
DOI  :  10.1186/2046-9063-8-7

 received in 2011-11-08, accepted in 2012-03-19,  发布年份 2012

【 摘 要 】

Background

The well-lit surface waters of oligotrophic gyres significantly contribute to global primary production. Marine cyanobacteria of the genus Prochlorococcus are a major fraction of photosynthetic organisms within these areas. Labile phosphate is considered a limiting nutrient in some oligotrophic regions such as the Caribbean Sea, and as such it is crucial to understand the physiological response of primary producers such as Prochlorococcus to fluctuations in the availability of this critical nutrient.

Results

Prochlorococcus strains representing both high light (HL) (MIT9312) and low light (LL) (NATL2A and SS120) ecotypes were grown identically in phosphate depleted media (10 μM P_i). The three strains displayed marked differences in cellular protein expression, as determined by high throughput large scale quantitative proteomic analysis. The only strain to demonstrate a significantly different growth rate under reduced phosphate conditions was MIT9312. Additionally, there was a significant increase in phosphate-related proteins such as PhoE (> 15 fold increase) and a depression of the Rubisco protein RbcL abundance in this strain, whereas there appeared to be no significant change within the LL strain SS120.

Conclusions

This differential response between ecotypes highlights the relative importance of phosphate availability to each strain and from these results we draw the conclusion that the expression of phosphate acquisition mechanisms are activated at strain specific phosphate concentrations.

【 授权许可】

   
2012 Fuszard et al; licensee BioMed Central Ltd.

【 图 表 】

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