Transcription factor binding sites are highly enriched within microRNA precursor sequences

Biology Direct

Jittima Piriyapongsa²  I King Jordan³  Andrew B Conley³  Tom Ronan⁴  Neil R Smalheiser¹ 

[1] Department of Psychiatry, University of Illinois at Chicago, UIC Psychiatric Institute MC912, 1601 W. Taylor Street, Chicago, IL 60612 USA

[2] Genome Institute, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Klong1, Klong Luang, Pathumthani 12120, Thailand

[3] School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332 USA

[4] Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, USA

关键词: drosha;    microRNA biogenesis;    Transcription factors;   

Others  :  797049
DOI  :  10.1186/1745-6150-6-61

 received in 2011-08-04, accepted in 2011-12-02,  发布年份 2011

【 摘 要 】

Background

Transcription factors are thought to regulate the transcription of microRNA genes in a manner similar to that of protein-coding genes; that is, by binding to conventional transcription factor binding site DNA sequences located in or near promoter regions that lie upstream of the microRNA genes. However, in the course of analyzing the genomics of human microRNA genes, we noticed that annotated transcription factor binding sites commonly lie within 70- to 110-nt long microRNA small hairpin precursor sequences.

Results

We report that about 45% of all human small hairpin microRNA (pre-miR) sequences contain at least one predicted transcription factor binding site motif that is conserved across human, mouse and rat, and this rises to over 75% if one excludes primate-specific pre-miRs. The association is robust and has extremely strong statistical significance; it affects both intergenic and intronic pre-miRs and both isolated and clustered microRNA genes. We also confirmed and extended this finding using a separate analysis that examined all human pre-miR sequences regardless of conservation across species.

Conclusions

The transcription factor binding sites localized within small hairpin microRNA precursor sequences may possibly regulate their transcription. Transcription factors may also possibly bind directly to nascent primary microRNA gene transcripts or small hairpin microRNA precursors and regulate their processing.

Reviewers

This article was reviewed by Guillaume Bourque (nominated by Jerzy Jurka), Dmitri Pervouchine (nominated by Mikhail Gelfand), and Yuriy Gusev.

【 授权许可】

   
2011 Piriyapongsa et al; licensee BioMed Central Ltd.

【 图 表 】

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