Morphological and immunohistochemical characterisation of seminomas in Norwegian dogs

Acta Veterinaria Scandinavica

Gjermund Gunnes³  Tom Grotmol¹  Ane Nødtvedt²  Tor Espen Thorvaldsen² 

[1]Institute of Population-Based Cancer Research, Cancer Registry of Norway, PO Box 5313, Majorstuen, 0304, Oslo, Norway

[2]Department of Companion Animal Clinical Sciences, Norwegian School of Veterinary Science, PO Box 8146 Dep, 0033, Oslo, Norway

[3]Department of Basic Sciences & Aquatic Medicine, Norwegian School of Veterinary Science, PO Box 8146 Dep, 0033, Oslo, Norway

关键词: Tumour;    Testis;    Seminoma;    Dog;   

Others  :  789448
DOI  :  10.1186/1751-0147-54-52

 received in 2012-05-08, accepted in 2012-09-03,  发布年份 2012

【 摘 要 】

Background

Seminomas in the dog have traditionally been assumed to resemble human spermatocytic seminomas, based on their low malignancy and high occurrence in old individuals. However, recently published studies indicate that canine seminomas can be classified as classical and spermatocytic seminomas in a similar way as in man, and that classical seminomas comprise a substantial proportion of seminomas in the dog. These two factors both contribute to increasing the potential of canine seminoma as a relevant model for human testicular cancer. The aim of the present study was to characterise seminoma in Norwegian dogs using morphology and immunohistochemistry, and determine whether these tumours are comparable with human classical seminoma.

Methods

By applying diagnostic criteria from human pathology, 45 seminomas from the Norwegian Canine Cancer Register were examined histologically with hematoxylin and eosin (HE) and periodic acid-Schiff (PAS) stains. All sections were stained immunohistochemically with antibodies against human placental alkaline phosphatase (PLAP) and the transmembrane receptor c-KIT.

Results

Although two of the seminomas showed immunohistochemical staining characteristics indicative of classical seminoma (PLAP+/c-KIT+), all 45 examined seminomas were morphologically consistent with spermatocytic seminoma.

Conclusions

The value of canine seminoma as a model for SE in man remains unclear. Among the 45 investigated tumours from Norwegian dogs, none were classified as classical seminoma based on morphological criteria consistent with human seminomas. Regional or breed differences in the occurrence of classical seminoma in the dog, as well as the lack of uniform diagnostic criteria, might explain the discrepancy between the findings in the current study and the results presented by other authors.

【 授权许可】

   
2012 Thorvaldsen et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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