Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa

Biotechnology for Biofuels

Feiyu Fan²  Guoli Ma²  Jingen Li²  Qian Liu¹  Johan Philipp Benz³  Chaoguang Tian¹  Yanhe Ma¹ 

[1] Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Dao32, Tianjin Airport Economic Area 300308, Tianjin, China

[2] University of Chinese Academy of Sciences, Yuquan Road, Beijing 100049, China

[3] Holzforschung München, TUM School of Life Sciences Weihenstephan, Technische Universität München, Hans-Carl-von-Carlowitz-Platz 2, Freising, Germany

关键词: RESS;    RNA-seq;    Lignocellulase secretion;    Transcription factor;    UPR;    ER stress;   

Others  :  1173377
DOI  :  10.1186/s13068-015-0248-5

 received in 2014-11-27, accepted in 2015-03-27,  发布年份 2015

【 摘 要 】

Background

Lignocellulolytic fungal cells suffer endoplasmic reticulum (ER) stress during lignocellulase synthesis; however, an understanding of this integrated process on a genome-wide scale remains poor. Here, we undertook a systematic investigation of this process in Neurospora crassa (N. crassa) using transcriptomic analysis coupled with genetic screens.

Results

A set of 766 genes was identified as the ER stress response targets (ESRTs) in N. crassa under cellulose utilization conditions. Among these, the expression of 223 and 186 genes showed dependence on IRE-1 and HAC-1, respectively. A total of 527 available mutants for ESRT genes were screened, 249 of which exhibited ER stress susceptibility, including 100 genes with unknown function. Disruption of ire-1 or hac-1 in N. crassa did not affect transcriptional induction of lignocellulase genes by cellulose but severely affected secretion of the corresponding enzymes. A global investigation of transcription factors (TFs) discovered three novel regulators (RES-1, RES-2, RRG-2) involved in lignocellulase secretion. Production of lignocellulases in Δres-1 increased by more than 30% in comparison to wild type (WT), while secretion decreased by nearly 30% in strains Δres-2 and Δrrg-2. Transcriptional profiling of the three TF mutants suggests they are deeply involved in lignocellulase secretion and ER stress response.

Conclusions

Here, we determined the transcriptional scope of the ER stress response during lignocellulase synthesis in the model cellulolytic fungus N. crassa. Through genome-wide mutant screening and analysis, dozens of novel genes were discovered to be involved in the process. The findings of this work will be useful for strain improvement to facilitate lignocellulase and biomass-based chemical production.

【 授权许可】

   
2015 Fan et al.; licensee BioMed Central.

【 图 表 】

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