Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions

Biotechnology for Biofuels

Magdalena Rakicka⁴  Zbigniew Lazar³  Thierry Dulermo²  Patrick Fickers¹  Jean Marc Nicaud⁴ 

[1] Microbial Processes and Interactions, Gembloux Agro Bio-Tech, Université de Liège, Passage des Déportés, 2, Gembloux, 5030, Belgium

[2] AgroParisTech, UMR Micalis, Jouy-en-Josas, France

[3] Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, Wrocław, 51-630, Poland

[4] Institut Micalis, INRA-AgroParisTech, UMR1319, Team BIMLip: Biologie Intégrative du Métabolisme Lipidique, CBAI, Thiverval-Grignon, 78850, France

关键词: Triglyceride;    Continuous culture;    Molasses;    Crude glycerol;    Biolipid production;    Oleaginous yeast;    Yarrowia lipolytica;   

Others  :  1225783
DOI  :  10.1186/s13068-015-0286-z

 received in 2015-04-14, accepted in 2015-07-10,  发布年份 2015

【 摘 要 】

Background

Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry.

Results

In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h ⁻¹ ; 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y _L/gly ) and the coefficient of lipid yield to biomass yield (Y _L/X) were equal to 0.1 and 0.4, respectively.

Conclusions

These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced.

【 授权许可】

   
2015 Rakicka et al.

【 图 表 】

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