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12 Enzyme evolution by in vitro compartmentalization In vitro compartmentalization (IVC) is based on water-in-oil emulsions where surfactants aid in the formation of microscopic aqueous compartments. These compartments simulate cellular compartments in which only the reaction for selection is performed. Inside each droplet transcription, translation, and expression of the desired protein takes place using active components. This method is poised to become the most promising for the future of enzyme evolution – we'll discuss why. Notes	88KB	PDF	download
12 Enzyme evolution by in vitro compartmentalization In vitro compartmentalization (IVC) is based on water-in-oil emulsions where surfactants aid in the formation of microscopic aqueous compartments. These compartments simulate cellular compartments in which only the reaction for selection is performed. Inside each droplet transcription, translation, and expression of the desired protein takes place using active components. This method is poised to become the most promising for the future of enzyme evolution – we'll discuss why. Notes	31KB	PDF	download

Directed Evolution: Engineering Biocatalysts
12 Enzyme evolution by in vitro compartmentalization In vitro compartmentalization (IVC) is based on water-in-oil emulsions where surfactants aid in the formation of microscopic aqueous compartments. These compartments simulate cellular compartments in which only the reaction for selection is performed. Inside each droplet transcription, translation, and expression of the desired protein takes place using active components. This method is poised to become the most promising for the future of enzyme evolution – we'll discuss why. Notes
Life Sciences Courses;Biological Engineering--Biomaterials;Biology--Cell Biology;Biology--Genetics
授课人:Dr. Kerry Love
机构:Massachusetts Institute of Technology

主题词:58.1
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