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Directed Evolution: Engineering Biocatalysts
8 Enzyme evolution using phage display (cont.) Affinity selection for improved catalysts has limitations; not all enzymatic activities have suitable suicide substrates. Direct selection of enzymes on the basis of catalysis is possible using phage display technology, but is more complex as a physical link between the phage-bound enzyme and the substrate must be established. We will discuss how this type of selection is achieved using two different examples of substrate linkage Notes
Life Sciences Courses;Biological Engineering--Biomaterials;Biology--Cell Biology;Biology--Genetics
授课人:Dr. Kerry Love
机构:Massachusetts Institute of Technology
主题词:58.1
美国|英语
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8 Enzyme evolution using phage display (cont.) Affinity selection for improved catalysts has limitations; not all enzymatic activities have suitable suicide substrates. Direct selection of enzymes on the basis of catalysis is possible using phage display technology, but is more complex as a physical link between the phage-bound enzyme and the substrate must be established. We will discuss how this type of selection is achieved using two different examples of substrate linkage Notes 89KB PDF download
8 Enzyme evolution using phage display (cont.) Affinity selection for improved catalysts has limitations; not all enzymatic activities have suitable suicide substrates. Direct selection of enzymes on the basis of catalysis is possible using phage display technology, but is more complex as a physical link between the phage-bound enzyme and the substrate must be established. We will discuss how this type of selection is achieved using two different examples of substrate linkage Notes 230KB PDF download
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