开放课件详细信息
| Directed Evolution: Engineering Biocatalysts | |
| 5 Enzyme evolution by genetic complementation In enzyme evolution, it is important to link the gene of interest, the enzyme it encodes and the product of the activity of the enzyme. Many methods exist for the selection of particular library members based on enzyme activity. We will begin be examining in vivo selection systems, which link cell survival to enzyme activity. Genetic complementation, the most basic of these selection systems, is the rescuing of a specific genetic defect leading to a particular phenotype by the addition of DNA encoding a gene of interest. Genetic complementation is often used to investigate the function of genes for which no function has previously been described. Notes | |
| Life Sciences Courses;Biological Engineering--Biomaterials;Biology--Cell Biology;Biology--Genetics | |
| 授课人:Dr. Kerry Love | |
| 机构:Massachusetts Institute of Technology | |
| 主题词:58.1 | |
| 美国|英语 | |
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| Files | Size | Format | View |
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| 5 Enzyme evolution by genetic complementation In enzyme evolution, it is important to link the gene of interest, the enzyme it encodes and the product of the activity of the enzyme. Many methods exist for the selection of particular library members based on enzyme activity. We will begin be examining in vivo selection systems, which link cell survival to enzyme activity. Genetic complementation, the most basic of these selection systems, is the rescuing of a specific genetic defect leading to a particular phenotype by the addition of DNA encoding a gene of interest. Genetic complementation is often used to investigate the function of genes for which no function has previously been described. Notes | 89KB |  download |
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| 5 Enzyme evolution by genetic complementation In enzyme evolution, it is important to link the gene of interest, the enzyme it encodes and the product of the activity of the enzyme. Many methods exist for the selection of particular library members based on enzyme activity. We will begin be examining in vivo selection systems, which link cell survival to enzyme activity. Genetic complementation, the most basic of these selection systems, is the rescuing of a specific genetic defect leading to a particular phenotype by the addition of DNA encoding a gene of interest. Genetic complementation is often used to investigate the function of genes for which no function has previously been described. Notes | 81KB | download |
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