Tissue Culture of Regeneration Induced by Leaves of 'Hongyang'

2018 3rd International Conference on Insulating Materials, Material Application and Electrical Engineering

材料科学;无线电电子学;电工学

Kong, Lingling^1 ; Song, Qinglin^1 ; Wang, Rui^1 ; Xie, Yutong^1 ; Zhang, Yong^1

College of Horticulture, Sichuan Agricultural University, Chengdu, China^1

关键词: 6 benzylaminopurine (6 BA);    Adventitious buds;    Comprehensive evaluation;    Media formulations;    Optimum combination;    Proliferation rate;    Survival ratio;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/452/2/022076/pdf
DOI  :  10.1088/1757-899X/452/2/022076

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

The test combined the concentration in 0.5mg⋅L-1, 1.0mg⋅L-1, 1.5mg⋅L-1 of 1-naphthaleneacetic acid (NAA) and a concentration of 2.0mg⋅L-1, 2.5mg⋅L-1, 3.0mg⋅L-1 of 6-benzylaminopurine (6-BA), from which we got a comprehensive evaluation of shoots survival ratio, growing point proliferated rate and shoots growth status together. From all the treatment we selected MS + 1.0mg⋅L-1 NAA + 3.0mg⋅L-1 6-BA for the optimum combination, for its live ratio of budding leaves on 92.59%, growing point proliferation rate on 629.60%. In this experiment, each treatment adventitious buds were over 300.00%, that all the treatments are able to use as the media formulations for proliferation of adventitious buds.