Adaptation of acid-tolerant Leucaena leucocephala cv Tarramba Calluses after gamma irradiation on regenerative media

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Latief, M.F.^1 ; Karti, P.D.M.H.^2 ; Prihantoro, I.^2

School of Postgraduate, Bogor Agricultural University, 16680, Indonesia^1

Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, 16680, Indonesia^2

关键词: Descriptive analysis;    Gamma irradiation;    Gamma-ray irradiation;    Gene mutations;    Leucaena leucocephala;    Plant breeding;    Randomized design;    Tolerant strains;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012120/pdf
DOI  :  10.1088/1757-899X/434/1/012120

来源: IOP

【 摘 要 】

The aim of this study to measure adaptation of callus Lamtoro (Leucaena leucocephala cv. Tarramba) after gamma irradiation on regenerative media. Lamtoro plant breeding in the form of callus with gamma ray irradiation is required for the development of a new plant-tolerant strain line with a gene mutation approach in tissue culture. The experimental design in this study was a complete randomized design (CRD). There were 13 treatments consitsing of control (M0). 6-benzyl amino purine (BAP) media (M1-M4). kinetin media (M5-M8). and combination of BAP and kinetin media (M9-M12) during 8 weeks. The measured variables were viability of callus. the addition of diameter and height callus. colour and texture callus. Data were tested using analysis of variance (ANOVA) and continued with Duncan test if there were a significant difference among treatment. Data of viability colour and texture used descriptive analysis. The data showed that formulation media M12 (combination 1 mg L-1 BAP and 1 mg L-1 kinetin) was significantly different (P