Application of thin layer chromatography-densitometry method for analysis of metoprolol in plasma and urine

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Utami, P.^1 ; Alfiyah, L.^1 ; Estika, D.^1

Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Jl. Raya Dukuhwaluh, Kembaran, Purwokerto, Central-Java

53182, Indonesia^1

关键词: Antihypertensive drugs;    Beta-blockers;    Correlation coefficient;    Densitometric measurement;    Densitometry;    Human plasmas;    Relative standard deviations;    Validation parameters;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012091/pdf
DOI  :  10.1088/1757-899X/434/1/012091

来源: IOP

【 摘 要 】

Metoprolol is one of the Beta blocker antihypertensive drugs. The aim of this study was to obtain a thin layer chromatography (TLC)-densitometry method for analysis of metoprolol in plasma and urine. The TLC was carried out on the aluminum sheet of silica gel 60 F254, using chloroform, methanol, and ammonia (4:6:0.2 v/v/v) as a mobile phase. Densitometric measurements were done using densitometer TLC Scanner 4 at 282 nm. The method showed good linearity with correlation coefficient (r) of 0.9899 (15-35 μg/spot) for plasma and 0.9904 (15-75 μg/spot) for urine. The minimum detectable amounts for plasma and urine were found to be 4.60 and 4.47 μg/spot respectively. Quantitative limits for plasma and urine were 15.34 and 14.90 μg/spot respectively. The accuracy of the method was accessed by % recovery and found to be 99.52 % for plasma and 102.73 % for urine. The relative standard deviation values for plasma and urine were 5.29 and 4.07% respectively. The method showed acceptable validation parameters. It can be used for the analysis of metoprolol in human plasma and urine.