Study on immobilization of papain with magnetic porous alginate microspheres

6th Annual International Conference on Material Science and Engineering

Wang, Hui^1 ; Shao, Dongxu^1

Hainan Marine Food Engineering Technology Center, Hainan Tropical Ocean University, Hainan Province Sanya

572022, China^1

关键词: Biomacromolecule;    Casein hydrolysis;    Efficient catalysts;    Industrial biocatalysis;    Porous polymer microspheres;    Porous structures;    Preparation procedures;    Residual activity;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/397/1/012031/pdf
DOI  :  10.1088/1757-899X/397/1/012031

来源: IOP

【 摘 要 】

Magnetic porous polymer microspheres were prepared by using biomacromolecule sodium alginate and magnetic Fe3O4 particles. Polyethylene glycol was involved in preparation procedure as a pore-forming agent.The microspheres were applied to immobilize papain via cross-linking method for catalysing the hydrolysis of protein. The formation of the porous structure improvedthe papain immobilization yield. In comparison to the free papain, the immobilized papain exhibited enhanced optimum pH, higher thermal stability and activity over the studied temperature 30°C -70°Cin casein hydrolysis. The immobilized papain retained nearly 72% of residual activity after repeating the casein hydrolysis reaction seven times. The apparent Km increased after papain immobilization.Magnetic porous alginate microspheres can be potentially used as a carrier for enzyme immobilization for constructing efficient catalysts for industrial biocatalysis.