Toxicity tests, antioxidant activity, and antimicrobial activity of chitosan

12th Joint Conference on Chemistry

Kurniasih, M.^1 ; Purwati^1 ; Dewi, R.S.^2

Chemistry Department, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Indonesia^1

Mycology and Phytopathology Laboratory, Faculty of Biology, Jenderal Soedirman University, Indonesia^2

关键词: 2 ,2-diphenyl-1-picrylhydrazyl;    Anti oxidative activity;    Anti-bacterial activity;    Anti-microbial activity;    Anti-microbial effects;    Anti-oxidant activities;    Brine shrimp lethalities;    Minimum inhibitory concentration;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/349/1/012037/pdf
DOI  :  10.1088/1757-899X/349/1/012037

来源: IOP

【 摘 要 】

Chitosan is a naturally occurring cationic biopolymer, obtained by alkaline deacetylation of chitin. This research aims to investigate the toxicity, antioxidant activity and antibacterial activity of chitosan from shrimp chitin. In this study, chitin extracted from shrimp waste material. Chitin is then deacetylation with 60% NaOH so that chitosan produced. Degrees of deacetylation, molecular weight, toxicity test, antioxidant activity and antimicrobial activity of chitosan then evaluated. Toxicity test using Brine Shrimp Lethality Test. The antioxidant analysis was performed using DPPH method (2, 2-diphenyl-1-picrylhydrazyl) and FTC method (ferric thiocyanate) in which the radical formed will reduce Ferro to Ferri resulting in a complex with thiocyanate. To determine the antibacterial activity of Staphylococcus aureus, antifungal in Candida albicans and Aspergillus niger by measuring antimicrobial effects and minimum inhibitory concentrations (MIC). Based on the result of research, the value of degrees of deacetylation, molecular weight, and LC50values of chitosan synthesis was 94,32, 1052.93 g/mol and 1364.41 ppm, respectively. In general, the antioxidative activities increased as the concentration of chitosan increased. MIC value of chitosan against S. aureus, C. albicans, and A. niger was 10 ppm, 15.6 ppm, and 5 ppm, respectively.