Acute and Subchronic Toxicity of Self Nanoemulsifying Drug Delivery Systems (SNEDDS) from Chloroform Bay Leaf Extract (Eugenia Polyantha W.) with Palm Kernel Oil as A Carrier

International Conference on Advanced Materials for Better Future 2017

Prihapsara, F.^1 ; Mufidah^1 ; Artanti, A.N.^1 ; Harini, M.^2

Department of Pharmacy, Faculty of Mathematics and Natural Science, SebelasMaret University, Indonesia^1

Department of Biology, Faculty of Mathematics and Natural Science, SebelasMaret University, Indonesia^2

关键词: Acute toxicity test;    Group animals;    Histopathological examinations;    Leaf extracts;    Median lethal dose;    Organ functions;    Palm kernel oil;    Self nanoemulsifying drug delivery system (SNEDDS);   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/333/1/012066/pdf
DOI  :  10.1088/1757-899X/333/1/012066

来源: IOP

【 摘 要 】

The present study was aimed to study the acute and subchronic toxicity of Self Nanoemulsifying Drug Delivery Systems (SNEDDS) from chloroform bay leaf extract with Palm Kernel Oil as carrier. In acute toxicity test, five groups of rat (n=5/groups) were orally treated with Self Nanoemulsifying Drug Delivery Systems (SNEDDS) from chloroform bay leaf extract with doses at 48, 240, 1200 and 6000 mg/kg/day respectively, then the median lethal dose LD50, advers effect and mortality were recorded up to 14 days. Meanwhile, in subchronic toxicity study, 4 groups of rats (n=6/group) received by orally treatment of SNEDDS from chloroform bay leaf extract with doses at 91.75; 183.5; 367 mg/kg/day respectively for 28 days, and biochemical, hematological and histopatological change in tissue such as liver, kidney, and pancreatic were determined. The result show that LD50is 1045.44 mg/kg. Although histopathological examination of most of the organs exhibited no structural changes, some moderate damage was observed in high dose group animals (367 mg/kg/day). The high dose of SNEDDS extract has shown mild signs of toxicity on organ function test.